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dc.contributor.authorChavez Rodriguez, Aldemar
dc.contributor.authorDubeibe, Diego
dc.contributor.authorberdugo, jesus
dc.date.accessioned2023-02-14T16:37:37Z
dc.date.available2023-02-14T16:37:37Z
dc.date.issued2023
dc.identifier.citationChavez-Rodriguez, A., Dubeibe-Marin, D. F., Crudeli, G., & Berdugo-Gutiérrez, J. A. (2023). In vitro production of buffalo embryos in colombia – results of the addition of growth factors and antioxidants to defined culture medium. [In vitro proizvodnja zametaka bizona u Kolumbiji - rezultati dodavanja faktora rasta i antioksidansa definiranom mediju za uzgoj zametaka] Veterinarska Stanica, 54(3), 293-299. doi:10.46419/vs.54.3.1spa
dc.identifier.issn0350-7149spa
dc.identifier.urihttps://repository.udca.edu.co/handle/11158/5128
dc.description.abstractTo increase the success rate of the in vitro production (IVP) system of embryos in buffalo, it is necessary to improve parameters. This field requires more research since its results are low compared to cattle. In this study carried out in Colombia, a modification of the methodology to produce buffalo IVP embryos is described, and 24 non-lactating females, aged 4 to 11 years old were included. Follicular wave was synchronised by ablation of the follicles within the ovary, seven days later all follicles were aspirated to obtain oocytes for maturation, fertilisation and embryo production. For all culture media used, animal protein was avoided. Culture media were supplemented with growth factors and other molecules associated with embryonic development. Special care was taken in adding supplements to protect the zygotes against the harmful effect of oxygen free radicals in the culture medium. Two aspiration series were performed 15 days apart. The average number of oocytes obtained by aspiration was 11.33, the number of blastocysts was 3.05, and the obtained blastocyst production rate (36.2%) was higher than literature reports for this species; we hypothesise that this increase is associated with the supplements added. It was possible to observe a female that produced 50 oocytes. Subsequent research is needed to evaluate the effect of the medium on embryo quality and pregnancy rates. A cost analysis of the new proposed medium is required, since all supplements must be imported and handled with a cold chain of up to -80oC, making it more expensive and restricting widespread use. Finally, it is shown that with three embryos per aspiration, at least one pregnancy can be successful after the aspiration, which is one of the objectives of the IVP systemeng
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.esspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://hrcak.srce.hr/279063spa
dc.titleIn vitro production of buffalo embryos in Colombia – results of the addition of growth factors and antioxidants to defined culture mediumeng
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.subject.lembBúfalos
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.description.notesIncluye referencias bibliográficasspa
dc.identifier.doihttps://doi.org/10.46419/vs.54.3.1
dc.subject.decsAntioxidantes
dc.subject.decsEstructuras Embrionarias
dc.subject.decsPéptidos y Proteínas de Señalización Intercelular
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.relation.indexedAgrosaviaspa
dc.relation.citationedition2023spa
dc.relation.citationendpage299spa
dc.relation.citationissue3spa
dc.relation.citationstartpage293spa
dc.relation.citationvolume54spa
dc.relation.ispartofjournalVeterinarska Stanicaspa
dc.type.contentTextspa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa


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