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dc.contributor.authorCamargo-Castañeda, Andrea M.
dc.contributor.authorStranahan, Lauren W.
dc.contributor.authorEdwards, John F.
dc.contributor.authorGarcia-Gonzalez, Daniel G.
dc.contributor.authorRoa, Leonardo
dc.contributor.authorAvila-Granados, Lisa M.
dc.contributor.authorHensel, Martha E.
dc.date.accessioned2021-02-05T16:55:54Z
dc.date.available2021-02-05T16:55:54Z
dc.date.issued2021
dc.identifier.citationCamargo-Castañeda, A. M., Stranahan, L. W., Edwards, J. F., Garcia-Gonzalez, D. G., Roa, L., Avila-Granados, L. M., . . . Arenas-Gamboa, A. M. (2021). Characterization of epididymal and testicular histologic lesions and use of immunohistochemistry and PCR on formalin-fixed tissues to detect brucella canis in male dogs. Journal of Veterinary Diagnostic Investigation, doi:10.1177/1040638720986883Es_es
dc.identifier.urihttps://journals.sagepub.com/doi/10.1177/1040638720986883
dc.description.abstractIn male dogs, Brucella canis frequently causes epididymitis, ultimately resulting in testicular atrophy and infertility. Although B. canis predominantly affects the epididymis, the misleading term “orchitis” is still commonly used by clinicians. Of additional concern, diagnosis in dogs remains challenging because of variable sensitivity and specificity of serologic assays and fluctuations in bacteremia levels in infected dogs, reducing the sensitivity of blood culture. We describe here the histologic lesions in the scrotal contents of 8 dogs suspected of being infected with B. canis and clinically diagnosed with orchitis. We explored the possibility of using immunohistochemistry (IHC) and real-time PCR (rtPCR) in formalin-fixed, paraffin-embedded (FFPE) tissues to detect the presence of B. canis. Epididymitis of variable chronicity was identified in all 8 dogs, with only 3 also exhibiting orchitis. Using rtPCR, the presence of B. canis was identified in 4 of 8 dogs, with 3 of these 4 dogs also positive by IHC. These results suggest that rtPCR and IHC are promising techniques that can be used in FFPE tissues to detect B. canis when other detection techniques are unavailable. Additionally, accurate recognition of epididymitis rather than orchitis in suspect cases could aid in accurate diagnosiseng
dc.format.mimetypeapplication/pdfEs_es
dc.language.isoengEs_es
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.eseng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/Es_es
dc.subject.meshBrucella canis
dc.subject.meshInmunohistoquímica
dc.subject.meshReacción en Cadena en Tiempo Real de la Polimerasa
dc.titleCharacterization of epididymal and testicular histologic lesions and use of immunohistochemistry and PCR on formalin-fixed tissues to detect Brucella canis in male dogseng
dc.typeArtículo de revistaEs_es
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessEs_es
dc.identifier.doi10.1177/1040638720986883
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)Es_es
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1Es_es
dc.type.driverinfo:eu-repo/semantics/articleEs_es
dc.type.versioninfo:eu-repo/semantics/publishedVersionEs_es
dc.relation.indexedScopusEs_es
dc.identifier.instnameUniversidad de Ciencias Aplicadas y AmbientalesEs_es
dc.identifier.reponameUDCAEs_es
dc.identifier.repourlrepository.udca.edu.coEs_es
dc.relation.citationedition(Ene. 19, 2021)Es_es
dc.relation.citationendpage5Es_es
dc.relation.citationstartpage1Es_es
dc.relation.ispartofjournalJournal of Veterinary Diagnostic InvestigationEs_es
dc.type.contentTextEs_es
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTEs_es
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2Es_es
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Es_es


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