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dc.contributor.authorCuy-Chaparro, Laura
dc.contributor.authorBohórquez, Michel David
dc.contributor.authorArevalo-Pinzon, Gabriela
dc.contributor.authorCastañeda-Ramírez, Jeimmy Johana
dc.contributor.authorSuárez, Carlos F.
dc.date.accessioned2021-02-05T14:23:39Z
dc.date.available2021-02-05T14:23:39Z
dc.date.issued2021
dc.identifier.citationCuy-Chaparro, L., Bohórquez, M. D., Arévalo-Pinzón, G., Castañeda-Ramírez, J. J., Suárez, C. F., Pabón, L., . . . Patarroyo, M. A. (2021). Babesia bovis ligand-receptor interaction: AMA-1 contains small regions governing bovine erythrocyte binding. International Journal of Molecular Sciences, 22(2), 1-15. doi:10.3390/ijms22020714Es_es
dc.identifier.urihttps://www.mdpi.com/1422-0067/22/2/714
dc.description.abstractApical membrane antigen 1 is a microneme protein which plays an indispensable role during Apicomplexa parasite invasion. The detailed mechanism of AMA-1 molecular interaction with its receptor on bovine erythrocytes has not been completely defined in Babesia bovis. This study was focused on identifying the minimum B. bovis AMA-1-derived regions governing specific and high-affinity binding to its target cells. Different approaches were used for detecting ama-1 locus genetic variability and natural selection signatures. The binding properties of twelve highly conserved 20-residue-long peptides were evaluated using a sensitive and specific binding assay based on radio-iodination. B. bovis AMA-1 ectodomain structure was modelled and refined using molecular modelling software. NetMHCIIpan software was used for calculating B-and T-cell epitopes. The B. bovis ama-1 gene had regions under functional constraint, having the highest negative selective pressure intensity in the Domain I encoding region. Interestingly, B. bovis AMA-1-DI (100YMQKFDIPRNHGSGIYVDLG119 and120GYESVGSKSYRMPVGKCPVV139 ) and DII (302CPMHPVRDAIFGKWSGGSCV321 )-derived peptides had high specificity interaction with erythrocytes and bound to a chymotrypsin and neuraminidase-treatment sensitive receptor. DI-derived peptides appear to be exposed on the protein’s surface and contain predicted B-and T-cell epitopes. These findings provide data (for the first-time) concerning B. bovis AMA-1 functional subunits which are important for establishing receptor-ligand interactions which could be used in synthetic vaccine development.eng
dc.format.mimetypeapplication/pdfEs_es
dc.language.isoengEs_es
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.eseng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/Es_es
dc.subject.meshMerozoítos
dc.subject.meshPlasmodium vivax
dc.subject.meshVacunas contra la Malaria
dc.subject.meshBabesia bovis
dc.titleBabesia Bovis Ligand-Receptor Interaction: AMA-1 Contains Small Regions Governing Bovine Erythrocyte Bindingeng
dc.typeArtículo de revistaEs_es
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessEs_es
dc.identifier.doi10.3390/ijms22020714
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)Es_es
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1Es_es
dc.type.driverinfo:eu-repo/semantics/articleEs_es
dc.type.versioninfo:eu-repo/semantics/publishedVersionEs_es
dc.relation.indexedScopusEs_es
dc.identifier.instnameUniversidad de Ciencias Aplicadas y AmbientalesEs_es
dc.identifier.reponameUDCAEs_es
dc.identifier.repourlrepository.udca.edu.coEs_es
dc.relation.citationedition(Jun. 2, 2021) Artículo No. 714Es_es
dc.relation.citationendpage15Es_es
dc.relation.citationissue2Es_es
dc.relation.citationstartpage1Es_es
dc.relation.citationvolume22Es_es
dc.relation.ispartofjournalInternational Journal of Molecular SciencesEs_es
dc.type.contentTextEs_es
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTEs_es
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2Es_es
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Es_es


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