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dc.contributor.authorGómez-Segura, Lidia
dc.contributor.authorParra, Alexander
dc.contributor.authorCalpena, Ana C.
dc.contributor.authorGimeno, Alvaro
dc.contributor.authorBoix-Montañes, Antonio
dc.date.accessioned2021-02-04T18:26:04Z
dc.date.available2021-02-04T18:26:04Z
dc.date.issued2020
dc.identifier.citationGómez-Segura, L., Parra, A., Calpena, A. C., Gimeno, Á., & Boix-Montañes, A. (2020). Carprofen permeation test through porcine ex vivo mucous membranes and ophthalmic tissues for tolerability assessments: Validation and histological study. Veterinary Sciences, 7(4), 1-17. doi:10.3390/vetsci7040152Es_es
dc.identifier.urihttps://www.mdpi.com/2306-7381/7/4/152
dc.description.abstractCarprofen (CP), a non-steroidal anti-inflammatory drug (NSAID), is profusely used in veterinary medicine for its analgesic and anti-inflammatory activity. Some undesirable effects are associated with its systemic administration. Alternative local routes are especially useful to facilitate its administration in animals. The main aim of this paper is to validate the suitability of ex vivo permeation experiments of CP with porcine mucous membranes (buccal, sublingual and vaginal) and ophthalmic tissues (cornea, sclera and conjunctiva) intended to be representative of naïve in vivo conditions. Chromatographic analysis of CP in membrane-permeated samples and drug-retained have been validated following standard bioanalytical guidelines. Then, recovery levels of drugs in tissue samples were assessed with aqueous phosphate buffered saline (PBS) buffer to preserve the histological integrity. Finally, as a proof of concept, a series of CP permeation tests in vertical Franz diffusion cells has been performed to evaluate permeation flux and permeability constants in all tissues, followed by a histological study for critical evaluation. Furthermore, synthetic tissue retention-like samples were prepared to verify the value of this experimental study. Results show linear relationships with good determination coefficient (R2 > 0.998 and R2 > 0.999) in the range of 0.78 to 6.25 mg/mL and 3.125 mg/mL to 100 mg/mL, respectively. Low limits of quantification around 0.40 µg/mL were allowed to follow permeation levels until a minimum of 0.40% of the locally-applied dose. This method showed a good accuracy and precision with values lower than 2%. After the recovery technique, reproducible values below 30% were achieved in all tissues, suggesting it is a non-damaging method with low efficiency that requires the use of further solvents to enhance the extraction percentages. After permeation and histology tests, no relevant peak interferences were detected, and no cell or tissue damage was found in any tissue. In conclusion, results demonstrate the suitability of this test to quantify the distribution of CP with good histological tolerability.eng
dc.format.mimetypeapplication/pdfEs_es
dc.language.isoengEs_es
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.esspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/Es_es
dc.subject.meshCromatografía Líquida de Alta Presión
dc.subject.meshBuprenorfina
dc.subject.meshMucosa Bucal
dc.subject.meshConjuntiva
dc.titleCarprofen Permeation Test through Porcine Ex VivoMucous Membranes and Ophthalmic Tissues forTolerability Assessments: Validation andHistological Studyeng
dc.typeArtículo de revistaEs_es
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessEs_es
dc.subject.lembCarprofeno
dc.identifier.doi10.3390/vetsci7040152
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)Es_es
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1Es_es
dc.type.driverinfo:eu-repo/semantics/articleEs_es
dc.type.versioninfo:eu-repo/semantics/draftEs_es
dc.relation.indexedScopusEs_es
dc.identifier.instnameUniversidad de Ciencias Aplicadas y AmbientalesEs_es
dc.identifier.reponameUDCAEs_es
dc.identifier.repourlrepository.udca.edu.coEs_es
dc.relation.citationedition(2020) Artículo No. 152Es_es
dc.relation.citationendpage17Es_es
dc.relation.citationissue4Es_es
dc.relation.citationstartpage1Es_es
dc.relation.citationvolume7Es_es
dc.relation.ispartofjournalVeterinary SciencesEs_es
dc.type.contentTextEs_es
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTEs_es
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2Es_es
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Es_es


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