Please use this identifier to cite or link to this item: https://repository.udca.edu.co/handle/11158/3497
Title: Strong -LAMP Assay Based on a Strongyloides spp.-Derived Partial Sequence in the 18S rRNA as Potential Biomarker for Strongyloidiasis Diagnosis in Human Urine Samples
Authors: Fernández Soto, Pedro
Celis Giraldo, Carmen Teresa
Collar Fernandez, Coralina
Gorgojo, Óscar
Camargo, Milena
Muñoz, José
Salas Coronas, Joaquín
Patarroyo, Manuel A.
Muro, Antonio
Issue Date: 2020
Citation: Fernández-Soto, P., Celis-Giraldo, C. T., Collar-Fernández, C., Gorgojo, Ó., Camargo, M., Muñoz, J., . . . Muro, A. (2020). Strong -LAMP assay based on a strongyloides spp.-derived partial sequence in the 18S rRNA as potential biomarker for strongyloidiasis diagnosis in human urine samples. Disease Markers, 2020 doi:10.1155/2020/5265198
Series/Report no.: Disease Markers;Vol. 2020, 2020, Article number 5265198 páginas 9 -10
Abstract: Human strongyloidiasis a soil-transmitted infection caused by Strongyloides stercoralis is one of the most neglected amongst the so-called Neglected Tropical Diseases (NTDs). S. stercoralis is a nematode, which is distributed worldwide; it has been estimated that it could affect millions of people, mainly in tropical and subtropical endemic regions. The difficulties of diagnosis lead to infection rates being underreported. Asymptomatic patients have chronic infections that can lead to severe hyperinfection syndrome or disseminated strongyloidiasis in immunocompromised patients. Strongyloidiasis can easily be misdiagnosed because conventional faecal-based techniques lack of sensitivity for the morphological identification of infective larvae in faeces. None of the currently used molecular methods have used urine samples as an alternative to faecal samples for diagnosing strongyloidiasis. This study was thus aimed at comparing, for the first time, the use of a new loop-mediated isothermal amplification (LAMP) molecular assay (Strong-LAMP) to traditional methods on patients’ urine samples. Twenty-four urine samples were taken from patients included in a study involving two Spanish hospitals for strongyloidiasis screening using parasitological and serological tests. Strongyloides larvae were found in 11 patients’ faecal samples, thereby ascertaining that they had the disease. Other patients had high antibody titres but no larvae were found in their faeces. All urine samples were analysed by PCR and Strong-LAMP assay. No amplification occurred when using PCR. Strong-LAMP led to detecting S. stercoralis DNA in urine samples from patients having previously confirmed strongyloidiasis by parasitological tests and/or a suspicion of being infected by serological ones. The Strong-LAMP assay is a useful molecular tool for research regarding strongyloidiasis in human urine samples. After further validation, the Strong-LAMP assay could also be used for complementary and effective diagnosis of strongyloidiasis in a clinical setting.
URI: https://www.hindawi.com/journals/dm/2020/5265198
Appears in Collections:CCB. Artículos indexados en Scopus

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