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dc.contributor.authorBernal Ulloa, Sandra Milenaspa
dc.contributor.authorHeinzmann, Juliaspa
dc.contributor.authorHerrmann, Dorisspa
dc.contributor.authorTimmermann, Berndspa
dc.contributor.authorBaulain, Ulrichspa
dc.contributor.authorGroßfeld, Rudolfspa
dc.contributor.authorDiederich, Mikespa
dc.date.accessioned2020-04-02T20:24:55Zspa
dc.date.available2020-04-02T20:24:55Zspa
dc.date.issued2013-04spa
dc.identifier.citationBernal, S. M., Heinzmann, J., Herrmann, D., Timmermann, B., Baulain, U., Grobfeld, R., . . . Niemann, H. (2013). Effects of different oocyte retrieval and in vitro maturation systems on bovine embryo development and quality. Zygote, 23(3), 367-377. doi:10.1017/S0967199413000658spa
dc.identifier.urihttps://www.cambridge.org/core/journals/zygote/article/effects-of-different-oocyte-retrieval-and-in-vitro-maturation-systems-on-bovine-embryo-development-and-quality/588A770E071EF38CAAD298962428EA69spa
dc.description.abstractCyclic adenosine monophosphate (cAMP) modulators have been used to avoid spontaneous oocyte maturation and concomitantly improve oocyte developmental competence. The current work evaluated the effects of the addition of cAMP modulators forskolin, 3-isobutyl-1-methylxanthine (IBMX) and cilostamide during in vitro maturation on the quality and yields of blastocysts. The following experimental groups were evaluated: (i) slicing or (ii) aspiration and maturation in tissue culture medium (TCM)199 for 24 h (TCM24slicing and TCM24aspiration, respectively), (iii) aspiration and maturation in the presence of cAMP modulators for 30 h (cAMP30aspiration) and in vivo-produced blastocysts. In vitro-matured oocytes were fertilized and presumptive zygotes were cultured in vitro to assess embryo development. Cleavage, blastocyst formation, blastocyst cell number, mRNA abundance of selected genes and global methylation profiles were evaluated. Blastocyst rate/zygotes for the TCM24aspiration protocol was improved (32.2 ± 2.1%) compared with TCM24slicing and cAMP30aspiration (23.4 ± 1.2% and 23.3 ± 2.0%, respectively, P 0.05), while those from the other groups were significantly elevated. It is concluded that retrieval, collection systems and addition of cAMP modulators can affect oocyte developmental competence, which is reflected not only in blastocyst rates but also in global DNA methylation and gene expression patterns.eng
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.relation.ispartofseriesZygote;Vol. 23, No. 3, Abr. 2013, páginas 367-377spa
dc.rightsDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleEffects of different oocyte retrieval and in vitro maturation systems on bovine embryo development and qualityeng
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.identifier.doihttps://doi.org/10.1017/S0967199413000658spa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.proposalPreimplantation embryospa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.subject.agrovocAMPspa
dc.subject.agrovocFosfatos (ésteres)spa
dc.subject.agrovocAdenosinaspa
dc.subject.agrovocÓvulospa
dc.type.contentTextspa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa


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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
Except where otherwise noted, this item's license is described as Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales