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Title: Effects of different oocyte retrieval and in vitro maturation systems on bovine embryo development and quality
Authors: Bernal Ulloa, Sandra Milena
Heinzmann, Julia
Herrmann, Doris
Timmermann, Bernd
Baulain, Ulrich
Großfeld, Rudolf
Diederich, Mike
Issue Date: Apr-2013
Citation: Bernal, S. M., Heinzmann, J., Herrmann, D., Timmermann, B., Baulain, U., Grobfeld, R., . . . Niemann, H. (2013). Effects of different oocyte retrieval and in vitro maturation systems on bovine embryo development and quality. Zygote, 23(3), 367-377. doi:10.1017/S0967199413000658
Series/Report no.: Zygote;Vol. 23, No. 3, Abr. 2013, páginas 367-377
Abstract: Cyclic adenosine monophosphate (cAMP) modulators have been used to avoid spontaneous oocyte maturation and concomitantly improve oocyte developmental competence. The current work evaluated the effects of the addition of cAMP modulators forskolin, 3-isobutyl-1-methylxanthine (IBMX) and cilostamide during in vitro maturation on the quality and yields of blastocysts. The following experimental groups were evaluated: (i) slicing or (ii) aspiration and maturation in tissue culture medium (TCM)199 for 24 h (TCM24slicing and TCM24aspiration, respectively), (iii) aspiration and maturation in the presence of cAMP modulators for 30 h (cAMP30aspiration) and in vivo-produced blastocysts. In vitro-matured oocytes were fertilized and presumptive zygotes were cultured in vitro to assess embryo development. Cleavage, blastocyst formation, blastocyst cell number, mRNA abundance of selected genes and global methylation profiles were evaluated. Blastocyst rate/zygotes for the TCM24aspiration protocol was improved (32.2 ± 2.1%) compared with TCM24slicing and cAMP30aspiration (23.4 ± 1.2% and 23.3 ± 2.0%, respectively, P 0.05), while those from the other groups were significantly elevated. It is concluded that retrieval, collection systems and addition of cAMP modulators can affect oocyte developmental competence, which is reflected not only in blastocyst rates but also in global DNA methylation and gene expression patterns.
Appears in Collections:CCB. Artículos indexados en Scopus

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