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dc.contributor.authorMéndez Callejas, Gina Marcelaspa
dc.contributor.authorLeone, Stefanospa
dc.contributor.authorTanzarella, Caterinaspa
dc.contributor.authorAntoccia, Antoniospa
dc.date.accessioned2020-04-01T20:09:33Zspa
dc.date.available2020-04-01T20:09:33Zspa
dc.date.issued2014spa
dc.identifier.citationMéndez-Callejas, G. M., Leone, S., Tanzarella, C., & Antoccia, A. (2014). Combretastatin a-4 induces p53 mitochondrial-relocalisation independent-apoptosis in non-small lung cancer cells. Cell Biology International, 38(3), 296-308. doi:10.1002/cbin.10199spa
dc.identifier.urihttps://udca.elogim.com:2782/doi/abs/10.1002/cbin.10199spa
dc.description.abstractCombretastatinA-4(CA-4)isoneofthemosteffectiveagentsusedinchemotherapy.Nevertheless,thecontributionofp53and Bim proteins in the CA-4-induced apoptosis in non-small lung cancer cells (NSCLC) remains unresolved, specifically on involving of p53 in the mitochondrial pathway activation by a transcription-independent mechanism. In this context, the p53null H1299 and wt-p53 H460 NSCLC cells, in the absence and presence of pifithrin-m (PFTm), an inhibitor of p53 mitochondrial-translocation, were treated with CA-4 and different cellular endpoints were analysed. In contrast to previous observationsinH460cells,CA-4failedintheactivationofanapoptoticresponseinH1299cells,thusindicatinganinvolvement of p53 in the cell death induced by the drug. We found that CA-4 led to p53 cellular re-localisation in H460 cells; in particular, p53 was released from the microtubular network and accumulated at mitochondria where it interacts with Bim protein and other proteins of the Bcl-2 (B-cell leukaemia-2) family, leading to cytochrome c release, alteration in the mitochondrial membrane polarisation, cell cycle arrest at the G2/M-phase, and cell death. Interestingly, the cytosolic and the mitochondrial accumulation of protein Bim was strictly dependent on p53 status. The extent of cell death was not reduced in H460 after combinedtreatmentofPFTmwithCA-4.Overall,thedatasupportamodelofCA-4-inducedapoptosisinNSCLC,forwhichthe expression of p53 protein is essential, but its mitochondrial function, linked to p53-transcription independent apoptosis pathway, is negligible.eng
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.relation.ispartofseriesCell Biology International;Vol. 38, No. 3, 2014, páginas 1-13spa
dc.rightsDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleCombretastatin A-4 induces p53 mitochondrial-relocalisation independent-apoptosis in non-small lung cancer cellsspa
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.identifier.doidoi: 10.1002/cbin.10199spa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.proposalApoptosisspa
dc.subject.proposalBim proteinspa
dc.subject.proposalCombretastatin A-4spa
dc.subject.proposalMitochondrial p53spa
dc.subject.proposalNon-small-cell lung carcinomaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.subject.agrovocTerapéutica medicamentosaspa
dc.subject.agrovocProteínasspa
dc.subject.agrovocCélulasspa
dc.subject.agrovocNeoplasmasspa
dc.type.contentTextspa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa


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