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dc.contributor.authorTafur Gómez, Gabriel A.spa
dc.contributor.authorPatarroyo Salcedo, Joaquín Hernánspa
dc.contributor.authorVargas, Marlene Izabel V.spa
dc.contributor.authorAraújo, Leandro S.spa
dc.contributor.authorFidelis, Cintia F.spa
dc.contributor.authorPrates Patarroyo, Pablo A.spa
dc.contributor.authorCortes Vecino, Jesus Alfredospa
dc.contributor.authorPortela, Ricardo Wagnerspa
dc.date.accessioned2020-03-14T13:00:32Zspa
dc.date.available2020-03-14T13:00:32Zspa
dc.date.issued2020spa
dc.identifier.citationTafur-Gómez, G. A., Patarroyo Salcedo, J. H., Vargas, M. I., Araújo, L., Fidelis, C. F., Prates-Patarroyo, P. A., . . . Portela, R. W. (2020). Intestinal changes and performance parameters in ticks feeding on calves immunized with subunits of immunogens against rhipicephalus microplus. Experimental and Applied Acarology, 80(1), 91-107. doi:10.1007/s10493-019-00451-8spa
dc.identifier.urihttps://www.scopus.com/search/form.uri?display=basicspa
dc.description.abstractWe describe the intestinal changes and biological parameters of the tick species Rhipicephalus microplus exposed to the immune response of calves vaccinated with two subunits of immunogens. The first group of Bos taurus calves was immunized with a synthetic peptide (SBm7462), whereas the second group received an inoculum for synthetic control. The third group was immunized with a recombinant peptide (rSBm7462); an inoculum was injected into a fourth group of calves for recombinant control. Each formulation was administered to these calves during three times at intervals of 30 days. At 21 days after the last immunization, the calves were challenged using a total of 4500 larvae per animal. Indirect ELISA was realized to identify the kinetics of IgGs from samples of calves studied. Naturally detaching ticks were collected for analyses of biological performance and histological changes in the midgut. We dissected randomly detached ticks. The midgut of each of these ticks was removed and processed routinely for histology, stained with hematoxylin-eosin (H&E) and slow Giemsa. Slides were also subjected to immunohistochemistry. The antibody response showed significant induction of high-affinity IgGs in calves immunized with both peptides in comparison to calves of the control groups. Histological changes included damage of the intestinal epithelium in ticks fed on immunized hosts and intense immunostaining in midgut cells, using the serum of calves immunized with recombinant peptide. There were significant differences in all biological performing parameters of ticks detached from vaccinated calves in comparison with ticks of the control groups. We identified reductions of 87.7 and 93.5% in engorged ticks detached from calves immunized with a synthetic and recombinant peptides, respectively, a 28 and 8.60% lower egg mass in groups immunized with synthetic and recombinant peptides, respectively, and a 38.4% reduction of the value of nutrient index/tick in the group immunized with the recombinant peptide. Our findings show that the immune response induced by small peptides in cattle can modify the digestion and metabolism of ticks fed on vaccinated animals, resulting in changes in tick performance.eng
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.relation.ispartofseriesExperimental and Applied Acarology;Vol.80, No.1, Ene 1. 2020 páginas 91-107spa
dc.rightsDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://www.scopus.com/search/form.uri?display=basicspa
dc.sourcehttps://www.scopus.com/search/form.uri?display=basicspa
dc.titleIntestinal changes and performance parameters in ticks feeding on calves immunized with subunits of immunogens against Rhipicephalus microplusspa
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.identifier.doi10.1007/s10493-019-00451-8spa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.proposalCattle tickspa
dc.subject.proposalHistologyspa
dc.subject.proposalImmunogenspa
dc.subject.proposalIxodidaespa
dc.subject.proposalMidgutspa
dc.subject.proposalTick controlspa
dc.subject.proposalTick proteinsspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.subject.agrovocMetastigmataspa
dc.subject.agrovocRhipicephalusspa
dc.subject.agrovocHistología animalspa
dc.subject.agrovocIxodidaespa
dc.subject.agrovocProteínasspa
dc.subject.agrovocBovinaspa
dc.subject.agrovocBos taurusspa
dc.subject.agrovocGanadospa
dc.relation.indexedAgriculturaspa
dc.type.contentTextspa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa


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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
Except where otherwise noted, this item's license is described as Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales