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dc.contributor.advisorOrdóñez, Diego Armando,
dc.contributor.authorVásquez Vásquez, David Alejandrospa
dc.description9 páginasspa
dc.description.abstractThe major histocompatibility class II complex (MHC-II) play an important effector role for 7 the adaptive immune response against extracellular pathogens. One of the most popular 8 advances for understanding the antigen presentation biological issues is based on the study 9 of the immunopeptidomes, characterizing the peptide repertoire for different the major 10 histocompatibility complex (MHC) allelic variants expressed by professional antigen 11 presenting cells such as macrophages and dendritic cells, allowing the identification of 12 vaccine candidates or therapeutic targets. The present study aimed to evaluate the expression 13 of the MHC-II molecules in bovine mononuclear phagocytic cells infected with two clinical 14 relevant bacteria, determining the best treatment conditions for further studies. We found the 15 differential expression of CD14+ and CD19- on these populations considering different 16 maturation conditions. Interestingly, the phagocytosis and MCH-II expression presented an 17 inverse behavior, obtaining the highest phagocytosis percentages from 2 to 8 hours after 18 infection and the maximum MCH-II expression levels in cells with more than 24 hours of 19 maturation, reducing their phagocytic activity, thus considering a multiplicity of infection MOI) of 1:40 (**P <0.01). Based on this observations, we established the optimal culture 21 conditions for future biological studies in novel areas such as immunopeptidomics.eng
dc.publisherBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2019spa
dc.rightsDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesspa
dc.titleCaracterización de la expresión del complejo mayor de histocompatibilidad clase II en monocitos y macrófagos derivados de monocitos bovinos infectados con dos bacterias de relevancia clínicaspa
dc.typeTrabajo de grado - Pregradospa
dc.description.notesIncluye bibliografíaspa
dc.identifier.localMVZ025 V17e 2019 (205718)spa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.agrovocGanado bovinospa
dc.subject.agrovocEnfermedades de los animalesspa
dc.subject.agrovocEnfermedades bacterianasspa
dc.description.degreenameMédico(a) Veterinario Zootecnistaspa
dc.publisher.facultyFacultad de Ciencias Agropecuariasspa
dc.publisher.programMedicina Veterinaria y Zootecniaspa

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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
Except where otherwise noted, this item's license is described as Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales