|Title:||TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria|
|Authors:||Alba, Martha P.|
Suarez, Carlos F.
Patarroyo, Manuel A.
Patarroyo, Manuel Elkin
|Citation:||Alba, M. P., Suarez, C. F., Varela, Y., Patarroyo, M. A., Bermudez, A., & Patarroyo, M. E. (2016). TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria. Biochemical and Biophysical Research Communications, 477(4), 654-660. doi:10.1016/j.bbrc.2016.06.115|
|Series/Report no.:||Biochemical and Biophysical Research Communications;Vol.477, No.4, Sep 2 2016 páginas 654-660|
|Abstract:||Fully-protective, long-lasting, immunological (FPLLI) memory against Plasmodium falciparum malaria regarding immune protection-inducing protein structures (IMPIPS) vaccinated into monkeys previously challenged and re-challenged 60 days later with a lethal Aotus monkey-adapted P. falciparum strain was found to be associated with preferential high binding capacity to HLA-DRβ1* allelic molecules of the major histocompatibility class II (MHC-II), rather than HLA-DRβ3*, β4*, β5* alleles. Complete PPIIL 3D structure, a longer distance (26.5 Å ± 1.5 Å) between residues perfectly fitting into HLA-DRβ1*PBR pockets 1 and 9, a gauche− rotamer orientation in p8 TCR-contacting polar residue and a larger volume of polar p2 residues was also found. This data, in association with previously-described p3 and p7 apolar residues having gauche+ orientation to form a perfect MHC-II-peptide-TCR complex, determines the stereo-electronic and topochemical characteristics associated with FPLLI immunological memory.|
|Appears in Collections:||CCB. Artículos indexados en Scopus|
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