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Title: Occurrence and molecular characterization of Giardia duodenalis in child population from Colombia
Authors: Avendaño, Catalina
Ramo, Ana
Vergara Castiblanco, Claudia
Bayona, Martín
Velasco Benitez, Carlos Alberto
Sánchez Acedo, Caridad
Quílez, Joaquín
Issue Date: 2019
Citation: Avendaño, C., Ramo, A., Vergara-Castiblanco, C., Bayona, M., Velasco-Benitez, C. A., Sánchez-Acedo, C., & Quílez, J. (2019). Occurrence and molecular characterization of giardia duodenalis in child population from colombia. Infection, Genetics and Evolution, 76 doi:10.1016/j.meegid.2019.104034
Series/Report no.: Infection, Genetics and Evolution;Vol.76, Dic 2019 Artículo 104034 páginas 1-5
Abstract: Giardia duodenalis is one of the most prevalent human intestinal parasite, with children living in developing countries being particularly at risk of infection. The occurrence and molecular diversity of G. duodenalis was investigated in stools specimens from 307 individuals aged one to nineteen years in Colombia. Samples were collected in three educational establishments (n: 163) and two hospital laboratories (n: 144) from urban and rural areas. Feces were concentrated using a biphasic sedimentation method and wet mounts of the sediment were examined by light microscopy. G. duodenalis assemblages and sub-assemblages were determined on positive samples by PCR of the triose phosphate isomerase (tpi), β-giardin (bg) and small-subunit (ssu) rRNA genes. G. duodenalis infection was detected by microscopy in 23 individuals (7.5%). The protozoan was more prevalent among specimens collected in educational establishments (11.6%) than in those obtained from hospital laboratories (2.8%). Infection was most common in individuals from urban areas and children aged 1–5 years. No significant association between diarrhea and infection could be demonstrated. Twenty Giardia-positive samples were successfully allocated to assemblage B (n: 11), sub-assemblage AII (n: 7), and assemblage A (n: 2). Results indicate the potential for transmission of G. duodenalis infection in children attending educational establishments and individuals from urban areas, where transmission seems to be primarily anthroponotic.
Appears in Collections:CCB. Artículos indexados en Scopus

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