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Title: Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography
Authors: Whitted, Crystal L.
Palau, Victoria E.
Torrenegra, Rubén D.
Rodrigez, Oscar E.
Harirforoosh, Sam
Issue Date: 2017
Citation: Whitted, C.L., Palau, V.E., Torrenegra, R.D. et al. Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography. BMC Res Notes 10, 29 (2017) doi:10.1186/s13104-016-2358-y
Series/Report no.: BMC Research Notes Vol. 10, No.1, Ene. 7 2017, Artículo No. 29
Abstract: Background: Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8-trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell lines (Thomas et al. in PLoS ONE 7:e39806, 5). Here, we present modified methods for the extraction and quantification of flavones A and B in rat colon tissue after intravenous dosing via high performance liquid chromatography, from the originally described procedure for extraction and quantification in rat plasma (Whitted et al. in J Chromatogr B Analyt Technol Biomed Life Sci 1001:150-155, 7). Results: Modifications included tissue homogenization (1 g tissue: 2 mL water), filtration of the supernatant with a PVDF membrane, and the use of only one calibration curve to determine the concentration of each flavone in colon tissue. Good separation was achieved and representative equations were linear with r 2 ≥ 0.99 for both flavones. Precision and accuracy for flavone A ranged from 0.88-24.03 and 109-116%. Precision and accuracy for flavone B ranged from 1.62-33.56 and 98-113%. Concentrations of 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g of flavone B were detected in rat colon tissue 6 h post dosing. Conclusions: Modifications to the extraction methods for flavone A and flavone B from rat colon tissue had good separation, precision, and accuracy
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