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dc.contributor.authorUrrego, R.spa
dc.contributor.authorBernal Uloa, S. M.spa
dc.contributor.authorChavarría, N. A.spa
dc.contributor.authorHerrera-Puerta, E.spa
dc.contributor.authorLucas-Hahn, A.spa
dc.contributor.authorHerrmann, D.spa
dc.contributor.authorWinkler, S.spa
dc.contributor.authorPache, D.spa
dc.contributor.authorNiemann, H.spa
dc.contributor.authorRodriguez-Osorio, N.spa
dc.date.accessioned2019-10-24T22:02:37Zspa
dc.date.available2019-10-24T22:02:37Zspa
dc.date.issued2017spa
dc.identifier.citationUrrego, R., Bernal-Ulloa, S., Chavarría, ,N.A., Herrera-Puerta, E., Lucas-Hahn, A., Herrmann, D., . . . Rodriguez-Osorio, N. (2017). Satellite DNA methylation status and expression of selected genes in bos indicus blastocysts produced in vivo and in vitro. Zygote, 25(2), 131-140. doi:http://dx.doi.org/10.1017/S096719941600040Xspa
dc.identifier.urihttps://www.scopus.com/search/form.uri?display=basicspa
dc.description.abstractBovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitantly the relative abundance of transcripts, critically involved in DNA methylation (DNMT1 and DNMT3A), growth and development (IGF2R) and pluripotency (POU5F1) in Bos indicus embryos produced in vitro or in vivo. Results revealed that methylation of BTS were higher (P < 0.05) in embryos produced in vitro compared with their in vivo produced counterparts, while the methylation status of BTαS was similar in both groups. There were no significant differences in transcript abundance for DNMT3A, IGF2R and POU5F1 between blastocysts produced in vivo and in vitro. However, a significantly lower amount of DNMT1 transcripts was found in the in vitro cultured embryos (P < 0.05) compared with their in vivo derived counterparts. In conclusion, this study reported only minor changes in the expression of developmentally important genes and satellite DNA methylation related to the in vitro embryo production system.eng
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.relation.ispartofseriesZygote;Vol. 25, No. 2 Abr. 1 2017 páginas 131-140spa
dc.rightsDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://udca.elogim.com:2092/record/display.uri?eid=2-s2.0-85011311208&origin=resultslist&sort=plf-f&src=s&nlo=1&nlr=20&nls=afprfnm-t&affilName=Universidad+de+Ciencias+Aplicadas+y+Ambientales&sid=ebaab1bcd4dbde71ac2479a686752d77&sot=afnl&sdt=cl&cluster=scopubyr%2c%222017%22%2ct&sl=67&s=%28AF-ID%28%22Universidad+de+Ciencias+Aplicadas+y+Ambientales%22+60106980%29%29&relpos=34&citeCnt=4&searchTerm=spa
dc.subject.meshCélulasspa
dc.subject.meshBovinosspa
dc.subject.meshBlastocitospa
dc.subject.meshADN (Citosina-5-) Metiltransferasaspa
dc.subject.meshMetilación de ADNspa
dc.subject.meshADN Satélitespa
dc.titleSatellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitrospa
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.identifier.doi10.1017/S096719941600040Xspa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.proposalBovinaespa
dc.subject.proposalEmbryonic Structuresspa
dc.subject.proposalCellsspa
dc.subject.proposalBlastocystspa
dc.subject.proposalDNA (Cytosine-5-)-Methyltransferasespa
dc.subject.proposalDNA Methylationspa
dc.subject.proposalDNA, Satellitespa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.subject.agrovocEstructuras embrionariasspa
dc.type.contentTextspa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa


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