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Title: Structural and functional characterization of toxic peptides purified from the venom of the Colombian scorpion Tityus macrochirus
Authors: Rincón Cortés, Clara Andrea
Olamendi Portugal, Timoteo
Carcamo Noriega, Edson Norberto
González Santillán, Edmundo
Zamudio Zuñiga, Frnando
Reyes Montaño, Edgar Antonio
Vega Castro, Nohora Angélica
Possani, Lourival Domingos
Issue Date: 2019
Citation: Rincón-Cortés, C.A., Olamendi-Portugal, T., Carcamo-Noriega, E.N., Santillán, E.G., Zuñiga, F.Z., Reyes-Montaño, E.A., Vega Castro, N.A., Possani, L.D. Structural and functional characterization of toxic peptides purified from the venom of the Colombian scorpion Tityus macrochirus (2019) Toxicon, 169, pp. 5-11.
Series/Report no.: Toxicon;Vol.169, Nov 2019 páginas 5-11
Abstract: The soluble venom of the scorpion Tityus macrochirus was separated by chromatographic procedures and three homogeneous peptides were obtained and their primary structures were determined. They were called: Tma1-Tma3, from the abbreviated name of the scorpion. Tma1 is a peptide containing 65 amino acids with four disulfide linkages and a molecular weight of 7386.2 Da. It is a mammalian toxin, shown to affect human sodium-channels sub-types hNav1.6 and hNav1.4. Tma2 and Tma3 are peptides containing 69 amino acids linked by four disulfide bonds, molecular weights 7819.7 and 7830.0 Da, respectively. They do not affect human sodium-channels but are lethal to insects (crickets). A phylogenic analysis of the three peptides and those of other toxic peptides isolated from the genus Tityus and Centruroides were grouped together and analyzed, permitting to obtain a topology with two main clades, one includes most sodium-channel anti-insect scorpion toxins and others includes mostly sodium-channel scorpion toxins anti-mammalian. Tma1 segregates among a group of well-studied β-class toxins of other Tityus species such as T. discrepans, T. obscurus and T. pachyurus. Tma2 and Tma3 are associated with anti-insect toxins, particularly with one of T. obscurus. This phylogenetic analysis confirms and enforces our experimental results obtained with these three new sodium-channel scorpion toxins.
Appears in Collections:CCB. Artículos indexados en Scopus

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