|Title:||Effects of BPA on expression of apoptotic genes and migration of ovine trophectoderm (oTr1) cells during the peri-implantation period of pregnancy|
|Authors:||Elmetwally, Mohammed A.|
Halawa, Amal A.
Lenis, Yaser Y.
Bazer, Fuller W.
|Citation:||Elmetwally, M.A., Halawa, A.A., Lenis, Y.Y., Tang, W., Wu, G., Bazer, F.W. Effects of BPA on expression of apoptotic genes and migration of ovine trophectoderm (oTr1) cells during the peri-implantation period of pregnancy (2019) Reproductive Toxicology, 83, pp. 73-79|
|Series/Report no.:||Reproductive Toxicology;Vol.83 Ene 2019 páginas 73-79|
|Abstract:||Bisphenol A (BPA) is an endocrine-disrupting chemical used in the manufacture of many products used daily. In the present study, the effects of BPA (1 × 10 −4 to 1 × 10 -9 M) on migration and on the expression of some apoptotic genes were examined in vitro using ovine trophectoderm (oTr1) primary cell line. The results revealed that BPA at 1 × 10 −9 , 1 × 10 −8 and 1 × 10 −7 M increased migration of oTr1 cells, while 1 × 10 −6 , 1 × 10 −5 and 1 × 10 −4 M BPA decreased cell migration. Regarding apoptosis, expression of the anti-apoptotic gene Bcl-2 mRNA was greater at 1 × 10 −8 and 1 × 10 −9 M BPA and was down-regulated at 1 × 10 −4 to 1 × 10 −7 M BPA; however, expression of pro-apoptotic genes (Bax, cathepsin B, caspase-3 and c-myc) was reduced at the higher concentrations of BPA. Results of this study suggest that BPA may impair implantation by decreasing migration of oTr1 cells and inhibiting apoptosis.|
|Appears in Collections:||CCB. Artículos indexados en Scopus|
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