Química del extracto Petrol de hojas de Muehlenbeckia tamnifolia (H.B.K.) Meisn.

Abstract

The study involved the identification of secondary metabolites present in the leaves of Muehlenbeckia tamnifolia (HBK) Meisner. We performed a total extract of petrol (petroleum ether) using a Soxhlet with a yield of 2.49%, the extract was performed column chromatography (CC), to separate the secondary metabolites present in these fractions, the fractions obtained were subjected to gas chromatography-mass spectrometry (GC-MS) for identification of metabolites was used Nist database 08, of the Universidad Distrital Francisco José de Caldas The petroleum ether extract of leaves of Muehlenbeckia tamnifolia Colombian species (HBK) Meisner identified the following compounds with their respective percentages of performance: Heptadecane 0.75%, Henicosano 2.67%, Hexatridecano 17.40%, 1-Heptacosanol 2.02%, Triacontano 2.15%, tetracontane 0.52%, Docosane 1.95%, 1-triacontanol 10.77%, 3,7,11,15-tetramethyl-2-hexadecen-1-ol 3.67%, hexacosanoato Methyl 4.73%, 1,2-Epoxinonadeno 17.20% , Alpha-tocopherol-beta-D-monosido 31.03%, 4-tert-butylcyclohexyl acetate 0.77%, (2Z)-2-Benzilenoctanal, benzyl benzoate 0.65%, Alpha-Tocopherol 3.98%, 1-Octacosanol 39.25%, L-(+) - ascorbic-2 ,6-dihexadecanoato 11.59%, 1 - Benzoic acid-2-ethylhexyl ester 1.72%, Estigamasterol 2.89%, 3-friedelanona 2.61%, 9,12,15 octadecatrienoic-acid 11.46%, 9-Tricosene 0.53%, 8-hexyl pentadecane 0.63%, 2,6,10,14 tetramethyl hexadecane 0.67%, 3-Methyl-5-propyl-nonane 0.51%, 9,12,15-octadecatrienoato acetate 3.05%, 1 Benzene,2-ester-diisoctil 87.24%, Hexadecanoato propyl 1.24%, 2-methyl hexadecanoic acid propyl ester 0.64%, 2 - (pentilmetileno)-octanal 0.86%, methyl 9,12,15-Octadecatrienoato 2.45% 2-phenyl-ethyl benzoate 2.01%, 19,12,15-propyl Octadecatrienoato 9.89%, 1.40-diol-tetracontane 3.70%, 2,6,10,14-tetramethyl pentadecane 0.58%, Dotriacontano 1.31%, 9,12,15-Butyl Octadecatrienoato 1.49%.

El estudio consistió en la identificación de los metabólitos secundarios presentes en las hojas de Muehlenbeckia tamnifolia (H.B.K.) Meisn. Se realizo un extracto total de petrol (éter de petróleo), empleando un equipo Soxhlet con un rendimiento del 2.49%, al extracto se le realizo cromatografía en columna (CC), con el fin de separar los metabólitos secundarios presentes en estas fracciones, a las fracciones obtenidas se les realizo cromatografía de gases acoplada a espectrometría de masas (CG-EM), para la identificación de los metabolitos se empleo la base de datos Nist 08, de la Universidad Distrital Francisco José de Caldas. En el extracto de éter de petróleo de las hojas de la especie colombiana Muehlenbeckia tamnifolia (H.B.K.) Meisn se identificaron los siguientes compuestos, con sus respectivos porcentajes de rendimiento: Heptadecano 0.75%, Henicosano 2.67%, Hexatridecano 17.40%, 1-Heptacosanol 2.02%, Triacontano 2.15%, Tetracontano 0.52%, Docosano 1.95%, 1-Triacontanol 10.77%, 3,7,11,15-tetrametil-2-hexadecen-1-ol 3.67%, Metil hexacosanoato 4.73%, 1,2-Epoxinonadeno 17.20%, Alfa-tocoferol-beta-D-monosido 31.03%, 4-ter-butilciclohexil acetato 0.77%, (2Z)-2-Benzilenoctanal, Benzil benzoato 0.65%, Alfa-Tocoferol 3.98%, 1-Octacosanol 39.25%, Acido L-(+)- ascorbico-2,6-dihexadecanoato 11.59%, 1- Acido Benzoico-2-etilhexil-ester 1.72%, Estigamasterol 2.89%, 3-friedelanona 2.61%, 9,12,15-Acido Octadecatrienoico 11.46%, 9-Tricoseno 0.53%, 8-hexil-Pentadecano 0.63%, 2,6,10,14-tetrametil hexadecano 0.67%, 3-Metil-5-propil-nonano 0.51%, 9,12,15-octadecatrienoato de etilo 3.05%,1,2-Benzeno-diisoctil ester 87.24%, Hexadecanoato de propil 1.24%, Acido hexadecanoico 2-metil-propil-ester 0.64%, 2-(pentilmetileno)-Octanal 0.86%, 9,12,15-Octadecatrienoato de metilo 2.45%, 2-fenil-etil benzoato 2.01%, 19,12,15-Octadecatrienoato de propilo 9.89%, 1.40-diol-tetracontano 3.70%, 2,6,10,14-Tetrametil pentadecano 0.58%, Dotriacontano 1.31%, 9,12,15-Octadecatrienoato de Butilo 1.49%.