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Uncertainties in projections of climate extremes indices in South America via Bayesian inference

Daniel Gouveia, Carolina | 2022

Historical simulations and projections of climate extremes indices of precipitation and temperature were analysed over South America until the end of the 21st century through 31 general circulation models (GCMs) under four Representative Concentration Pathways. Simulations were compared with reanalysis data, and a Bayesian inference method was used to assess the uncertainties involved in the multi-model climate projections. Regarding the precipitation extremes indices, the GCMs' simulations reasonably approached the reanalysis data, but with heterogeneous biases, both in sign and in the location of the highest values. The temperature extremes indices presented the smallest biases when compared to precipitation. Projections show a gradual growth of precipitation extremes events as the analysed radiative forcing scenario increases, both in magnitude and extent, over a large part of South America. Projections also indicate a decrease in cold days and nights and an increase in warm days and nights, more pronounced in the equatorial region. Bayesian inference method smoothed changes in precipitation extremes events, both in magnitude and extent, compared to the simple GCMs' ensemble mean. There was no considerable variation in the temperature indices when applying the Bayesian inference. Finally, the probability density functions resulted in a predominance of multimodal and wide curves for the precipitation indices, showing great uncertainties in the GCMs' results, differently from those for the temperature indices, where the GCMs presented good agreement represented through unimodal and narrow curves.

Methodological considerations in injury burden of disease studies across Europe: a systematic literature review

Charalampous, Periklis | 2022

Background: Calculating the disease burden due to injury is complex, as it requires many methodological choices. Until now, an overview of the methodological design choices that have been made in burden of disease (BoD) studies in injury populations is not available. The aim of this systematic literature review was to identify existing injury BoD studies undertaken across Europe and to comprehensively review the methodological design choices and assumption parameters that have been made to calculate years of life lost (YLL) and years lived with disability (YLD) in these studies. Methods: We searched EMBASE, MEDLINE, Cochrane Central, Google Scholar, and Web of Science, and the grey literature supplemented by handsearching, for BoD studies. We included injury BoD studies that quantified the BoD expressed in YLL, YLD, and disability-adjusted life years (DALY) in countries within the European Region between early-1990 and mid-2021. Results: We retrieved 2,914 results of which 48 performed an injury-specific BoD assessment. Single-country independent and Global Burden of Disease (GBD)-linked injury BoD studies were performed in 11 European countries. Approximately 79% of injury BoD studies reported the BoD by external cause-of-injury. Most independent studies used the incidence-based approach to calculate YLDs. About half of the injury disease burden studies applied disability weights (DWs) developed by the GBD study. Almost all independent injury studies have determined YLL using national life tables. Conclusions: Considerable methodological variation across independent injury BoD assessments was observed; differences were mainly apparent in the design choices and assumption parameters towards injury YLD calculations, implementation of DWs, and the choice of life table for YLL calculations. Development and use of guidelines for performing and reporting of injury BoD studies is crucial to enhance transparency and comparability of injury BoD estimates across Europe and beyond

Gender differences in adaptation to heat in Spain

Navas-Martín, M. A. | 2022

In Spain the average temperature has increased by 1.7 ◦C since pre-industrial times. There has been an increase in heat waves both in terms of frequency and intensity, with a clear impact in terms of population health. The effect of heat waves on daily mortality presents important territorial differences. Gender also affects these impacts, as a determinant that conditions social inequalities in health. There is evidence that women may be more susceptible to extreme heat than men, although there are relatively few studies that analyze differences in the vulnerability and adaptation to heat by sex. This could be related to physiological causes. On the other hand, one of the indicators used to measure vulnerability to heat in a population and its adaptation is the minimum mortality temperature (MMT) and its temporal evolution. The aim of this study was to analyze the values of MMT in men and women and its temporal evolution during the 1983–2018 period in Spain’s provinces. An ecological, longitudinal retrospective study was carried out of time series data, based on maximum daily temperature and daily mortality data corresponding to the study period. Using cubic and quadratic fits between daily mortality rates and the temperature, the minimum values of these functions were determined, which allowed for determining MMT values. Furthermore, we used an improved methodology that provided for the estimation of missing MMT values when polynomial fits were inexistent. This analysis was carried out for each year. Later, based on the annual values of MMT, a linear fit was carried out to determine the rate of evolution of MMT for men and for women at the province level. Average MMT for all of Spain’s provinces was 29.4 ◦C in the case of men and 28.7 ◦C in the case of women. The MMT for men was greater than that of women in 86 percent of the total provinces analyzed, which indicates greater vulnerability among women. In terms of the rate of variation in MMT during the period analyzed, that of men was 0.39 ◦C/decade, compared to 0.53 ◦C/decade for women, indicating greater adaptation to heat among women, compared to men. The differences found between men and women were statistically significant. At the province level, the results show great heterogeneity. Studies carried out at the local level are needed to provide knowledge about those factors that can explain these differences at the province level, and to allow for incorporating a gender perspective in the implementation of measures for adaptation to high temperatures.

Antimicrobial Activity Developed by Scorpion Venoms and Its Peptide Component

Rincón Cortes, Clara Andrea | 2022

Microbial infections represent a problem of great importance at the public health level, with a high rate of morbidity-mortality worldwide. However, treating the different diseases generated by microorganisms requires a gradual increase in acquired resistance when applying or using them against various antibiotic therapies. Resistance is caused by various molecular mechanisms of microorganisms, thus reducing their effectiveness. Consequently, there is a need to search for new opportunities through natural sources with antimicrobial activity. One alternative is using peptides present in different scorpion venoms, specifically from the Buthidae family. Different peptides with biological activity in microorganisms have been characterized as preventing their growth or inhibiting their replication. Therefore, they represent an alternative to be used in the design and development of new-generation antimicrobial drugs in different types of microorganisms, such as bacteria, fungi, viruses, and parasites. Essential aspects for its disclosure, as shown in this review, are the studies carried out on different types of peptides in scorpion venoms with activity against pathogenic microorganisms, highlighting their high therapeutic potential.

The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows

Sharawy, Heba A. | 2022

Aim OvSynch is a hormonal protocol for synchronization of estrus and use of artificial insemination (AI) at an optimal time without adverse effects on the ovaries or uterus. This study investigated the use of noninvasive color Doppler ultrasound to assess changes in uterine and vaginal blood flow during the Ovsynch program for synchronization of estrus and its relation to the pregnancy rates in Holstein cows. Materials and methods The experimental cows received an intramuscular dose of 10 μg of a GnRH analogue (G1), followed 7 days later with an intramuscular injection of synthetic prostaglandin F2α (P: PGF2α) analogue (500 μg cloprostenol sodium), and given a 10 μg, injection of the GnRH analogue (G2) i.m. 48 h after the PGF2α treatment, and the cows were bred 14-16 h after. Uterine and vaginal perfusion were investigated by performing transrectal Doppler ultrasonography of both the uterine and vaginal arteries in Holstein cows at different time points during the Ovsynch program to determine: peak systolic velocity (PSV), time-averaged maximum velocity (TAMV), the volume of blood flow (BFV), pulsatility index (PI), resistance index (RI), resistance impedance (S/D) and diameters of uterine (UA) and vaginal (VA) arteries. Steroid hormones were also assayed. Transrectal ultrasonography (TUS) was performed at 32 and 60 days to confirm the pregnancy per artificial insemination (P/AI). Results The uterine PSV, TAMV, and PV were greater at the time of the cloprostenol sodium and second GnRH injections (p<0.05) than at the time of the first GnRH injection. The vaginal PSV, PV were greater at the time of the cloprostenol sodium than at the time of the first and second GnRH injections (p<0.05). The receiver operating characteristic curve (ROC curve) indicated a high correlation between the uterine and vaginal blood flow and the rate of the pregnancy (p<0.05). The area under the ROC curve was 0.920 and 0.87 (p<0.05) for vaginal and uterine arteries respectively at time of G2. The serum levels of progesterone, estrogen and cortisol were correlated with the P/AI (p<0.05). The P/AI significantly decreased from 43.9 % at 32 d to 35.37 % at 60 d. Conclusion These results indicate that noninvasive Doppler ultrasonography is a valid method to evaluate changes in the characteristics of uterine and vaginal blood flow in cows during the Ovsynch protocol. Furthermore, vaginal and uterine blood flow are two determinant factors for the higher conception rates in Holstein dairy cows.

Evaluation of extreme precipitation climate indices and their projected changes for Brazil: From CMIP3 to CMIP6

Jeferson de Medeiros, Felipe | 2022

Extreme events usually cause numerous economic and social losses, especially in vulnerable countries, such as Brazil. Understanding whether the evolution of Earth System Models (ESMs) from Coupled Model Intercomparison Project (CMIP) improves the representation of extreme events and investigating their future change is fundamental because device policies of adaptation and mitigation to climate change generally consider the results of the most recent generation of ESMs. This study analyzes the performance of a subset of 40 ESMs from CMIP3, CMIP5, and CMIP6 in simulating eight extreme precipitation climate indices over Brazil during 1981–2000 and also estimates their projected changes for the middle (2046–2065) and far future (2081–2 100) under the worst-case scenario for each CMIP generation. Results reveal that CDD are the most challenging precipitation index to be simulated, while the best ones were PRCPTOT and R20mm. The model performance shows that CMIP3 has the best skill for Northeast Brazil, CMIP5 for Center-West, and CMIP6 for North, Southeast and South regions. Thus, at least for Brazil, the evolution of the ESMs from CMIP did not reflect a substantial improvement in the representation of precipitation climate extremes over all Brazilian regions. In addition, all the models across CMIP generations have difficulty in simulating the observed trends. This indicate that improvements are still needed in CMIP models. Despite the relative low performance in the historical climate, the climate projections indicate a consensus signal among most of precipitation climate extremes and CMIP generations, which increase its reliability. Overall, the extreme precipitation events are projected to be more severe, frequent, and long-lasting in all Brazilian regions, with the more pronounce changes expected in heavy rainfall and severe droughts in the central northern portion of Brazil and in the southern sector

A GBD 2019 study of health and Sustainable Development Goal gains and forecasts to 2030 in Spain

Lazarus, Jeffrey V. | 2022

This study aimed to report mortality, risk factors, and burden of diseases in Spain. The Global Burden of Disease, Injuries, and Risk Factors 2019 estimates the burden due to 369 diseases, injuries, and impairments and 87 risk factors and risk factor combinations. Here, we detail the updated Spain 1990–2019 burden of disease estimates and project certain metrics up to 2030. In 2019, leading causes of death were ischaemic heart disease, stroke, chronic obstructive pulmonary disease, Alzheimer’s disease, and lung cancer. Main causes of disability adjusted life years (DALYs) were ischaemic heart disease, diabetes, lung cancer, low back pain, and stroke. Leading DALYs risk factors included smoking, high body mass index, and high fasting plasma glucose. Spain scored 74/100 among all health-related Sustainable Development Goals (SDGs) indicators, ranking 20 of 195 countries and territories. We forecasted that by 2030, Spain would outpace Japan, the United States, and the European Union. Behavioural risk factors, such as smoking and poor diet, and environmental factors added a significant burden to the Spanish population’s health in 2019. Monitoring these trends, particularly in light of COVID-19, is essential to prioritise interventions that will reduce the future burden of disease to meet population health and SDG commitments.

The Black Box of Cellular and Molecular Events of Plasmodium vivax Merozoite Invasion into Reticulocytes

Molina-Franky, Jessica | 2022

Plasmodium vivax is the most widely distributed malaria parasite affecting humans worldwide, causing ~5 million cases yearly. Despite the disease’s extensive burden, there are gaps in the knowledge of the pathophysiological mechanisms by which P. vivax invades reticulocytes. In contrast, this crucial step is better understood for P. falciparum, the less widely distributed but more often fatal malaria parasite. This discrepancy is due to the difficulty of studying P. vivax’s exclusive invasion of reticulocytes, which represent 1–2% of circulating cells. Its accurate targeting mechanism has not yet been clarified, hindering the establishment of long-term continuous in vitro culture systems. So far, only three reticulocyte invasion pathways have been characterised based on parasite interactions with DARC, TfR1 and CD98 host proteins. However, exposing the parasite’s alternative invasion mechanisms is currently being considered, opening up a large field for exploring the entry receptors used by P. vivax for invading host cells. New methods must be developed to ensure better understanding of the parasite to control malarial transmission and to eradicate the disease. Here, we review the current state of knowledge on cellular and molecular mechanisms of P. vivax’s merozoite invasion to contribute to a better understanding of the parasite’s biology, pathogenesis and epidemiology.

Identification of Babesia bovis MSA-1 functionally constraint regions capable of binding to bovine erythrocytes

Cuy Chaparro, Laura | 2022

Merozoite surface antigen-1 is a glycoprotein expressed by Babesia bovis and is considered a vaccine candidate given that antibodies against it are able to partially block in vitro invasion of bovine erythrocytes. Despite this, no study to date has confirmed the target cell binding properties of the full MSA-1 or its fragments. This research has thus been focused on identifying protein regions playing a role in erythrocyte attachment, based on genetic diversity and natural selection analysis. Two regions under functional constraint (nucleotides 134–428 and 464–629) having a preponderance of negatively-selected signals were identified in silico. Three non-overlapping peptides derived from functionally constraint regions (42422 (39PEGSFYDDMSKFYGAVGSFD58), 42424 (91NALIKNNPMIRPDLFNATIV110) and 42426 (150TDIVEEDREKAVEYFKKHVY169)) were able to specifically bind to a sialoglycoprotein located on the bovine erythrocyte surface as confirmed by sensitive and specific peptide-cell interaction competition assays using both enzymatically treated and untreated red blood cells. Interestingly, it was predicted that peptides 42422 and 42426 have a helical structure and conserved motifs in all strain/isolates. These findings provide evidence, for the first time, related to B. bovis MSA-1 short regions used by the parasite in erythrocyte binding which could be predicted using natural selection analysis. Future work focused on evaluating these peptides’ antigenic ability during natural infection, and their ability to induce protection in immunisation assays are needed to confirm their usefulness as synthetic vaccine candidates.

Relationship between Dynamic and Isometric Strength, Power, Speed, and Average Propulsive Speed of Recreational Athletes

Fernandez, Jairo Alejandro | 2022

The purpose of this study was to examine the type of relationship between measures of maximal force (dynamic and isometric), maximal power, and mean propulsive velocity. In total, 355 recreational athletes, 96 women (age 20.5 2.5 years; height 158.2 17.3 cm; weight 61.8 48.4 kg) and 259 men (age 21.0 2.6 years; height 170.5 12.6 cm; weight 65.9 9.2 kg) were evaluated in three sessions separated by 72 h each in isometric midthigh pull exercise (ISOS) (kg), bench press maximum strength (1RM MSBP) (kg), jump height (CMJ) (m), and maximum pedaling power (WT) the maximum squat strength (1RM MSS) (kg), the mean propulsive velocity in the bench press (MPVBP) (m s�����1), and the peak power (PPBP) (w), mean propulsive squat velocity (MPVS) (m s�����1), peak power (PP) (w), maximum handgrip force (ISOHG) (kg), and 30 m movement speed (V30) (s). Significant correlations (p 0.01) were identified between 95% of the various manifestations of force, and only 5% presented a significance of p 0.05; however, when the magnitude of these correlations is observed, there is great heterogeneity. In this sense, the dynamic strength tests present the best correlations with the other strength and power tests used in the present study, followed by PPBP and PP. The results of this study complement what is reported in the literature regarding the correlation between different types of force manifestations being heterogeneous and contradictory.

Changes in the Cervical Microbiota of Women with Different High-Risk Human Papillomavirus Loads

Camargo, Milena | 2022

Abstract: The cervical microbiota is essential in female sexual health, and its altered states seem to have a central role in the dynamic of high-risk papillomavirus (hrHPV) infections. This study aimed to evaluate the variation in bacterial communities’ compositions according to hrHPV. We collected two samples per woman, with a difference of 12 1 months between them, and performed a follow-up on 66 of these women. The viral load (VL) of the hrHPV was estimated by quantitative PCR (qPCR), then it was normalized (using the HMBS gene as reference) and transformed to the Log10 scale to facilitate the interpretation. The VL was categorized as Negative, without hrHPV copies; Low, less than 100 hrHPV copies; Medium, between 100 to 102 hrHPV copies; and High, >102 hrHPV copies. The microbiota composition was described through the Illumina Novaseq PE250 platform. The diversity analyses revealed changes regarding the hrHPV VL, where women with low VL (<100 hrHPV copies) presented high diversity. The community state type (CST) IV was the most common. However, in women with high VL, a lower association with Lactobacillus depletion was found. Lactobacillus gallinarum and L. iners were the most abundant species in women with high VL, whereas women with low VL had a 6.06 greater probability of exhibiting Lactobacillus dominance. We identified conspicuous differences in the abundance of 78 bacterial genera between women with low and high VL, where 26 were depleted (e.g., Gardnerella) and 52 increased (e.g., Mycoplasma). A multilevel mixed-effects linear regression showed changes in the diversity due to the interaction between the measurement time and the VL, with a decrease in diversity in the second follow-up in women with low VL (Coeff. = 0.47), whereas the women with medium VL displayed an increase in diversity (Coeff. = 0.58). Here, we report for the first time that the cervical microbiota is influenced by the number of copies of hrHPV, where a decrease in the abundance of Lactobacillus, greater diversity, and enrichment of bacterial taxa is relevant in women with low VL.

Protein Supplementation during Mid-Gestation Alters the Amino Acid Patterns, Hepatic Metabolism, and Maternal Skeletal Muscle Turnover of Pregnant Zebu Beef Cows

Moreno Meneses, Javier Andrés | 2022

From 100 to 200 days of gestation, 52 cows carrying male (n = 30) or female (n = 22) fetuses were assigned to CON (basal diet—5.5% of CP, n = 26) or SUP (basal diet + protein supplement [40% CP, 3.5 g/kg BW]—12% of CP, n = 26) treatments. Glucose concentrations decreased at 200 (p ≤ 0.01; CON = 46.9 and SUP = 54.7 mg/dL) and 270 days (p ≤ 0.05; CON = 48.4 and SUP = 53.3 mg/dL) for CON compared to SUP. The same pattern occurred for insulin (p ≤ 0.01). At parturition, the NEFA concentration was greater (p = 0.01, 0.10 vs. 0.08 mmol/L) for CON than for SUP. Total AA increased in SUP (p ≤ 0.03) at mid- and late-gestation compared to CON. At 200 days, CON dams carrying females had less essential AA (p = 0.01) than cows carrying males. The SUP dams had greater expressions of protein synthesis markers, namely eIf4E and GSK3β (p ≤ 0.04), at day 200 and of MuFR1 (protein degradation marker, p ≤ 0.04) at parturition. Supplemented cows had higher hepatic pyruvate carboxylase expressions (p = 0.02). Therefore, PS alleviates the restriction overload on maternal metabolism.

Intranasal instillation of Pasteurella multocida lipopolysaccharide in rabbits causes interstitial lung damage

Patiño, Pilar | 2022

In order to characterize the in vivo lesions in the nasal cavities and lungs, twenty-eight rabbits were intranasally instilled with lipopolysaccharide (LPS) from P. multocida and then divided into seven groups according to euthanasia time. The nasal cavities and the lungs were processed for light microscopy, lectin histochemistry and transmission electron microscopy. Increased goblet cell activation and neutrophil infiltration were relevant changes in the nasal cavity. A predominantly interstitial pattern of diffuse alveolar damage and bronchopneumonic foci were the main lesions found in the lungs. LPS was found in the cytoplasm of ciliated cells, goblet cells, glandular cells, venular endothelial cells and neutrophils in the nasal cavity and in club cells, capillary endothelial cells and neutrophil in the lung. This study demonstrates that the LPS is able to cause lesions in the upper and lower respiratory tract, it binds to and is internalized by respiratory epithelial cells. Furthermore, it also traverses the intercellular spaces to reach the blood vessels, where it binds to and is internalized by neutrophil and red blood cells. These cells may then travel to the lungs where the LPS induces typical diffuse alveolar damage. This route of lung interstitial damage, to our knowledge, has not been described for this molecule or any known pathogen.

The InfAct proposal for a sustainable European health information infrastructure on population health: the Distributed Infrastructure on Population Health (DIPoH)

Sarmiento Suárez, Rodrigo | 2022

Background: In Europe, data on population health is fragmented, difficult to access, project-based and prone to health information inequalities in terms of availability, accessibility and especially in quality between and within countries. This situation is further exacerbated and exposed by the recent COVID-19 pandemic. The Joint Action on Health Information (InfAct) that builds on previous works of the BRIDGE Health project, carried out collaborative action to set up a sustainable infrastructure for health information in the European Union (EU). The aim of this paper is to present InfAct’s proposal for a sustainable research infrastructure, the Distributed Infrastructure on Population Health (DIPoH), which includes the setup of a Health Information Portal on population health to be maintained beyond InfAct’s time span. Methods: The strategy for the proposal was based on three components: scientific initiatives and proposals to improve Health Information Systems (HIS), exploration of technical acceptability and feasibility, and finally obtaining high-level political support. The technical exploration (Technical Dialogues—TD) was assumed by technical experts proposed by the countries, and political guidance was provided by the Assembly of Members (AoM), which gathered representatives from Ministries of Health and Science of EU/EEA countries. The results from the AoM and the TD were integrated in the sustainability plan compiling all the major outputs of InfAct. Results: The InfAct sustainability plan was organized in three main sections: a proposal of a new research infrastructure on population health (the DIPoH), new health information tools and innovative proposals for HIS, and a comprehensive capacity building programme. These activities were carried out in InfAct and are being further developed in the Population Health Information Research Infrastructure (PHIRI). PHIRI is a practical rollout of DIPoH facilitating and generating the best available evidence for research on health and wellbeing of populations as impacted by COVID-19. Conclusions: The sustainability plan received wide support from Member States and was recognized to have an added value at EU level. Nevertheless, there were several aspects which still need to be considered for the near future such as: (i) a commitment of stable financial and political support by Member States (MSs), (ii) the availability of resources at regional, national and European level to deal with innovations, and (iii) a more direct involvement from EU and international institutions such as the European Centre for Disease Prevention and Control (ECDC), the World Health Organization (WHO) and the Organisation for Economic Cooperation and Development OECD for providing support and sustainable contributions.

Selection indexes to optimise genetic and economic progress in Colombian Blanco Orejinegro cattle

Amaya, Alejandro | 2022

The use of selection indexes optimises genetic and economic gains in a population. The objective of this study was to propose and compare selection indexes built with economic and statistical methodologies in Colombian Creole Blanco Orejinegro breed. Genetic, phenotypic and economic information of the breed were used to design and test the selection indexes. The weights of each selection criteria (weight at 24 months of age, age at first calving, calving interval) were estimated using phenotypic variances, eigenvectors from a principal component analysis and economic values obtained through a deterministic bio-economic model. The selection criteria with the highest weight was weight at 24 months of age, with values of 0.56 and 0.034 USD using eigenvectors and phenotypic variance, respectively. The calving interval had the highest weight using the bio-economic model, with a value of -1.3 USD. The greatest profit due to genetic selection was obtained using the economic values as weights, which increased the average expected profit to a value of 18.76 USD/cow/year. The selection indexes calculated from phenotypic and eigenvectors do not rank animals similarly to the economic selection index. It could generate less genetic and economic progress in the national selection programme for the Blanco Orejinegro breed.

Experimental intoxication of Brahman (Bos indicus) heifers with Enterolobium cyclocarpum fruits

Lozano, María C. | 2022

Enterolobium cyclocarpum is a poisonous plant distributed throughout the Americas. The E. cyclocarpum fruits have high toxic potential for cattle in Colombia and the clinical signs and pathological lesions are ill-defined. To begin address this issue, twelve Brahman heifers were administered E. cyclocarpum fruits and the evolution of clinical signs were recorded. Blood was collected to establish biochemical and hematological parameters. Animals were euthanized between 4 and 15 days after the initial dose was given, and tissue samples were routinely processed and stained by Hematoxylin-Eosin. The severity of clinical signs and tissue lesions were correlated with the dose of E. cyclocarpum fruits. Clinical signs included fever, tachypnea, sialorrhea, jaundice, tympanism, and diarrhea. Skin lesions were consistent with photosensitization. Hematological and biochemical tests showed increased hematocrit, neutropenia, increased serum fibrinogen, elevated hepatic enzymes and azotemia. Histology revealed panlobular cytoplasmic vacuolization and extensive foci of necrosis in the liver. The skin, fore-stomach, abomasum and intestine revealed microcirculatory, inflammatory and ulcerative changes. Protein casts and tubular epithelium vacuolization were found in kidney. Depending on the toxicosis intensity, it is concluded that E. cyclocarpum fruits may cause two clinical and pathological forms of poisoning in Brahman heifers. First, a severe intoxication at repeated exposition with high (20 g/kg/d) or low (10 g/kg/d) dose that affected the digestive and tegumentary systems and the kidney. Second, a mild to moderate form with a single low dose (10 g/kg/d) that affected in lower grades the same systems/organs.

Transcriptome profile in the skeletal muscle of cattle progeny as a function of maternal protein supplementation during mid-gestation

Carvalho, Elisa | 2022

This study aimed to evaluate the differentially expressed genes (DEG), their biological functions, and the main predictor genes in skeletal muscle of the offspring resulting from dams supplemented or not with protein during mid-gestation. From 100 to 200 days of gestation, 10 Tabapuã cows pregnant of males were assigned to one of two treatments: Control [(CON) - supply of basal diet achieving 5.5% of crude protein (CP); n = 6]; or Supplemented [(SUP) - basal diet plus a supplement with 40% of CP provided at the level of 3.5 g/kg of body weight; n = 4]. Muscle samples were collected from the progeny at 260d of postnatal age to RNA-seq, Gene Ontology (GO) enrichment, and Random Forest (RF) analyses. A total of 310 DEG (q-value < 0.05) were identified for the effect of diet, being 187 and 123 genes down-regulated and up-regulated in the SUP diet, respectively. Within the SUP up-regulated genes, were genes related to insulin receptor signaling pathway (LOC107131843) and apoptosis regulation (KRT18, KRT8 and KRT19). While the enriched GOs terms are implicated in mitochondrial metabolism through the overrepresentation of the GO terms microtubule polymerization or depolymerization (GO:0031109, GO:0046785) and positive regulation of cellular component biogenesis (GO:0044089). Moreover, RF analysis revealed predictor genes related to the skeletal muscle development, including genes that participates in the PI3K/Akt pathways (PITPNA), myoblast proliferation (ALKBH1) and differentiation (CDK5R1 and miR-206). In conclusion, maternal protein supplementation during mid-gestation positively affected the energy metabolism and favored the hypertrophic processes of the skeletal muscle of the offspring.

Climate extremes and their impacts on agriculture across the Eastern Corn Belt Region of the U.S.

Wilson, Aaron B. | 2022

The Eastern Corn Belt Region (ECBR) is an important agricultural sector for the U.S. This study analyzes the climate extremes over the contemporary (1980–2018) and future (2036–2099) periods over the ECBR. We evaluated the performance of 32 downscaled models from the U.S. Global Change Research Program's Localized Constructed Analogs (LOCA) of the Coupled Model Intercomparison Project (CMIP5) to simulate extreme temperature and precipitation indices. The LOCA downscaled models were evaluated for the recent past against the National Aeronautics and Space Administration (NASA)-supported gridded observational dataset DAYMET. Results reveal key trends throughout the region that are consistent with previous studies, including significant increases in extreme minimum temperatures, reduction of cold nights, increase of warm nights, and decreases in diurnal temperature ranges. Much of the region demonstrates extreme warming trends in the coldest night of the year (more than 5 °C) and an increase in the heaviest precipitation events over 1980–2018. An optimal model ensemble (OME) was constructed using a Kling-Gupta Efficiency and Bhattacharyya coefficient evaluation to construct a comprehensive ranking procedure. Having outperformed a standard multi-model ensemble approach, the OME was used to evaluate the future changes of extreme climate indices under RCP4.5 and RCP8.5 scenarios. Though the OME showed consistently strong warming throughout the ECBR, variability among the optimal models and across watersheds is quite significant, especially for precipitation indices. Thus, constraining the uncertainty in future climate models, specifically as it relates to agriculture decisions that support climate-resilience, remains a challenge.

Acute coronary syndromes following COVID-19 vaccine application: Kounis syndrome or chance?

Rueda Ibarra, Leonardo | 2022

Agroforestry systems recover tree carbon stock faster than natural succession in Eastern Amazon, Brazil

Gómez Cardozo, Ernesto | 2022

The potential of agroforestry systems (AFS) for atmospheric carbon sequestration in degraded tropical lands is of key interest for climate change and rural development policies. This study evaluated aboveground and soil (0–20 cm) carbon stocks of AFS, secondary forests (SF), conserved and logged mature forests, on 88 sites in the eastern Brazilian Amazon. Tree carbon stock was higher in young (< 10 years) and advanced (> 30 years) AFS (10.2 ± 2.0 and 47.2 ± 8.1 Mg ha−1, respectively) when compared to the same age SF (5.8 ± 2.5 and 26.5 ± 19.5 Mg ha−1). However, aboveground and total carbon stocks were statistically similar within the same age categories of AFS and SF, because shrub pool were higher in SF. Conserved mature forests had the highest carbon stocks (190.2 ± 11.0 Mg ha−1), and carbon stocks in logged mature forests (119.4 ± 5.1 Mg ha−1) were similar to the advanced stages of AFS (108.6 ± 7.5 Mg ha−1). Litter and soil organic carbon (SOC) did not differ significantly between land-use systems nor along succession. At 30 years, aboveground carbon recovery was 46% (± 16) in AFS and 35% (± 21) in SF. Vegetation structural diversity (measured by DBH and height variation) was a good predictor of aboveground carbon stocks. Our results show the potential of AFS for carbon recovery, especially in the tree pool at late stages of development. Structurally more complex AFS provide an alternative to recover degraded lands and to develop synergies between climate change mitigation, adaptation, and goods production in Amazon.