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Intranasal instillation of Pasteurella multocida lipopolysaccharide in rabbits causes interstitial lung damage

Patiño, Pilar | 2022

In order to characterize the in vivo lesions in the nasal cavities and lungs, twenty-eight rabbits were intranasally instilled with lipopolysaccharide (LPS) from P. multocida and then divided into seven groups according to euthanasia time. The nasal cavities and the lungs were processed for light microscopy, lectin histochemistry and transmission electron microscopy. Increased goblet cell activation and neutrophil infiltration were relevant changes in the nasal cavity. A predominantly interstitial pattern of diffuse alveolar damage and bronchopneumonic foci were the main lesions found in the lungs. LPS was found in the cytoplasm of ciliated cells, goblet cells, glandular cells, venular endothelial cells and neutrophils in the nasal cavity and in club cells, capillary endothelial cells and neutrophil in the lung. This study demonstrates that the LPS is able to cause lesions in the upper and lower respiratory tract, it binds to and is internalized by respiratory epithelial cells. Furthermore, it also traverses the intercellular spaces to reach the blood vessels, where it binds to and is internalized by neutrophil and red blood cells. These cells may then travel to the lungs where the LPS induces typical diffuse alveolar damage. This route of lung interstitial damage, to our knowledge, has not been described for this molecule or any known pathogen.

The InfAct proposal for a sustainable European health information infrastructure on population health: the Distributed Infrastructure on Population Health (DIPoH)

Sarmiento Suárez, Rodrigo | 2022

Background: In Europe, data on population health is fragmented, difficult to access, project-based and prone to health information inequalities in terms of availability, accessibility and especially in quality between and within countries. This situation is further exacerbated and exposed by the recent COVID-19 pandemic. The Joint Action on Health Information (InfAct) that builds on previous works of the BRIDGE Health project, carried out collaborative action to set up a sustainable infrastructure for health information in the European Union (EU). The aim of this paper is to present InfAct’s proposal for a sustainable research infrastructure, the Distributed Infrastructure on Population Health (DIPoH), which includes the setup of a Health Information Portal on population health to be maintained beyond InfAct’s time span. Methods: The strategy for the proposal was based on three components: scientific initiatives and proposals to improve Health Information Systems (HIS), exploration of technical acceptability and feasibility, and finally obtaining high-level political support. The technical exploration (Technical Dialogues—TD) was assumed by technical experts proposed by the countries, and political guidance was provided by the Assembly of Members (AoM), which gathered representatives from Ministries of Health and Science of EU/EEA countries. The results from the AoM and the TD were integrated in the sustainability plan compiling all the major outputs of InfAct. Results: The InfAct sustainability plan was organized in three main sections: a proposal of a new research infrastructure on population health (the DIPoH), new health information tools and innovative proposals for HIS, and a comprehensive capacity building programme. These activities were carried out in InfAct and are being further developed in the Population Health Information Research Infrastructure (PHIRI). PHIRI is a practical rollout of DIPoH facilitating and generating the best available evidence for research on health and wellbeing of populations as impacted by COVID-19. Conclusions: The sustainability plan received wide support from Member States and was recognized to have an added value at EU level. Nevertheless, there were several aspects which still need to be considered for the near future such as: (i) a commitment of stable financial and political support by Member States (MSs), (ii) the availability of resources at regional, national and European level to deal with innovations, and (iii) a more direct involvement from EU and international institutions such as the European Centre for Disease Prevention and Control (ECDC), the World Health Organization (WHO) and the Organisation for Economic Cooperation and Development OECD for providing support and sustainable contributions.

Selection indexes to optimise genetic and economic progress in Colombian Blanco Orejinegro cattle

Amaya, Alejandro | 2022

The use of selection indexes optimises genetic and economic gains in a population. The objective of this study was to propose and compare selection indexes built with economic and statistical methodologies in Colombian Creole Blanco Orejinegro breed. Genetic, phenotypic and economic information of the breed were used to design and test the selection indexes. The weights of each selection criteria (weight at 24 months of age, age at first calving, calving interval) were estimated using phenotypic variances, eigenvectors from a principal component analysis and economic values obtained through a deterministic bio-economic model. The selection criteria with the highest weight was weight at 24 months of age, with values of 0.56 and 0.034 USD using eigenvectors and phenotypic variance, respectively. The calving interval had the highest weight using the bio-economic model, with a value of -1.3 USD. The greatest profit due to genetic selection was obtained using the economic values as weights, which increased the average expected profit to a value of 18.76 USD/cow/year. The selection indexes calculated from phenotypic and eigenvectors do not rank animals similarly to the economic selection index. It could generate less genetic and economic progress in the national selection programme for the Blanco Orejinegro breed.

Experimental intoxication of Brahman (Bos indicus) heifers with Enterolobium cyclocarpum fruits

Lozano, María C. | 2022

Enterolobium cyclocarpum is a poisonous plant distributed throughout the Americas. The E. cyclocarpum fruits have high toxic potential for cattle in Colombia and the clinical signs and pathological lesions are ill-defined. To begin address this issue, twelve Brahman heifers were administered E. cyclocarpum fruits and the evolution of clinical signs were recorded. Blood was collected to establish biochemical and hematological parameters. Animals were euthanized between 4 and 15 days after the initial dose was given, and tissue samples were routinely processed and stained by Hematoxylin-Eosin. The severity of clinical signs and tissue lesions were correlated with the dose of E. cyclocarpum fruits. Clinical signs included fever, tachypnea, sialorrhea, jaundice, tympanism, and diarrhea. Skin lesions were consistent with photosensitization. Hematological and biochemical tests showed increased hematocrit, neutropenia, increased serum fibrinogen, elevated hepatic enzymes and azotemia. Histology revealed panlobular cytoplasmic vacuolization and extensive foci of necrosis in the liver. The skin, fore-stomach, abomasum and intestine revealed microcirculatory, inflammatory and ulcerative changes. Protein casts and tubular epithelium vacuolization were found in kidney. Depending on the toxicosis intensity, it is concluded that E. cyclocarpum fruits may cause two clinical and pathological forms of poisoning in Brahman heifers. First, a severe intoxication at repeated exposition with high (20 g/kg/d) or low (10 g/kg/d) dose that affected the digestive and tegumentary systems and the kidney. Second, a mild to moderate form with a single low dose (10 g/kg/d) that affected in lower grades the same systems/organs.

Transcriptome profile in the skeletal muscle of cattle progeny as a function of maternal protein supplementation during mid-gestation

Carvalho, Elisa | 2022

This study aimed to evaluate the differentially expressed genes (DEG), their biological functions, and the main predictor genes in skeletal muscle of the offspring resulting from dams supplemented or not with protein during mid-gestation. From 100 to 200 days of gestation, 10 Tabapuã cows pregnant of males were assigned to one of two treatments: Control [(CON) - supply of basal diet achieving 5.5% of crude protein (CP); n = 6]; or Supplemented [(SUP) - basal diet plus a supplement with 40% of CP provided at the level of 3.5 g/kg of body weight; n = 4]. Muscle samples were collected from the progeny at 260d of postnatal age to RNA-seq, Gene Ontology (GO) enrichment, and Random Forest (RF) analyses. A total of 310 DEG (q-value < 0.05) were identified for the effect of diet, being 187 and 123 genes down-regulated and up-regulated in the SUP diet, respectively. Within the SUP up-regulated genes, were genes related to insulin receptor signaling pathway (LOC107131843) and apoptosis regulation (KRT18, KRT8 and KRT19). While the enriched GOs terms are implicated in mitochondrial metabolism through the overrepresentation of the GO terms microtubule polymerization or depolymerization (GO:0031109, GO:0046785) and positive regulation of cellular component biogenesis (GO:0044089). Moreover, RF analysis revealed predictor genes related to the skeletal muscle development, including genes that participates in the PI3K/Akt pathways (PITPNA), myoblast proliferation (ALKBH1) and differentiation (CDK5R1 and miR-206). In conclusion, maternal protein supplementation during mid-gestation positively affected the energy metabolism and favored the hypertrophic processes of the skeletal muscle of the offspring.

Climate extremes and their impacts on agriculture across the Eastern Corn Belt Region of the U.S.

Wilson, Aaron B. | 2022

The Eastern Corn Belt Region (ECBR) is an important agricultural sector for the U.S. This study analyzes the climate extremes over the contemporary (1980–2018) and future (2036–2099) periods over the ECBR. We evaluated the performance of 32 downscaled models from the U.S. Global Change Research Program's Localized Constructed Analogs (LOCA) of the Coupled Model Intercomparison Project (CMIP5) to simulate extreme temperature and precipitation indices. The LOCA downscaled models were evaluated for the recent past against the National Aeronautics and Space Administration (NASA)-supported gridded observational dataset DAYMET. Results reveal key trends throughout the region that are consistent with previous studies, including significant increases in extreme minimum temperatures, reduction of cold nights, increase of warm nights, and decreases in diurnal temperature ranges. Much of the region demonstrates extreme warming trends in the coldest night of the year (more than 5 °C) and an increase in the heaviest precipitation events over 1980–2018. An optimal model ensemble (OME) was constructed using a Kling-Gupta Efficiency and Bhattacharyya coefficient evaluation to construct a comprehensive ranking procedure. Having outperformed a standard multi-model ensemble approach, the OME was used to evaluate the future changes of extreme climate indices under RCP4.5 and RCP8.5 scenarios. Though the OME showed consistently strong warming throughout the ECBR, variability among the optimal models and across watersheds is quite significant, especially for precipitation indices. Thus, constraining the uncertainty in future climate models, specifically as it relates to agriculture decisions that support climate-resilience, remains a challenge.

Acute coronary syndromes following COVID-19 vaccine application: Kounis syndrome or chance?

Rueda Ibarra, Leonardo | 2022

Agroforestry systems recover tree carbon stock faster than natural succession in Eastern Amazon, Brazil

Gómez Cardozo, Ernesto | 2022

The potential of agroforestry systems (AFS) for atmospheric carbon sequestration in degraded tropical lands is of key interest for climate change and rural development policies. This study evaluated aboveground and soil (0–20 cm) carbon stocks of AFS, secondary forests (SF), conserved and logged mature forests, on 88 sites in the eastern Brazilian Amazon. Tree carbon stock was higher in young (< 10 years) and advanced (> 30 years) AFS (10.2 ± 2.0 and 47.2 ± 8.1 Mg ha−1, respectively) when compared to the same age SF (5.8 ± 2.5 and 26.5 ± 19.5 Mg ha−1). However, aboveground and total carbon stocks were statistically similar within the same age categories of AFS and SF, because shrub pool were higher in SF. Conserved mature forests had the highest carbon stocks (190.2 ± 11.0 Mg ha−1), and carbon stocks in logged mature forests (119.4 ± 5.1 Mg ha−1) were similar to the advanced stages of AFS (108.6 ± 7.5 Mg ha−1). Litter and soil organic carbon (SOC) did not differ significantly between land-use systems nor along succession. At 30 years, aboveground carbon recovery was 46% (± 16) in AFS and 35% (± 21) in SF. Vegetation structural diversity (measured by DBH and height variation) was a good predictor of aboveground carbon stocks. Our results show the potential of AFS for carbon recovery, especially in the tree pool at late stages of development. Structurally more complex AFS provide an alternative to recover degraded lands and to develop synergies between climate change mitigation, adaptation, and goods production in Amazon.

La enfermería latinoamericana y su resignificación social y cultural

Velásquez Jiménez, Claudia Marcela | 2022

El artículo aborda una reflexión sobre la importancia del liderazgo y empoderamiento de la enfermería Latinoamericana como cuerpo colectivo, que tiene una responsabilidad en la participación y construcción de la política pública de salud y su talento humano, a partir de la visibilizacion del aporte que, como disciplina fundamentada en investigación y teoría, realiza al conocimiento basado en evidencia del cuidado de los proceso de salud enfermedad de los individuos familias y colectivos. Plantea, además, la problemática de escasez de profesionales de enfermería en la región, la inequidad en el reconocimiento de su valor como profesión del cuidado humano y la necesidad de acción y protagonismo para enfrentar los desafíos de una real re- significación de la profesión.

Bar Load-Velocity Profile of Full Squat and Bench Press Exercises in Young Recreational Athletes

Fernandez, Jairo Alejandro | 2022

The purpose of this study was to determine the mean propulsive velocity (MVP) at various percentages of one repetition maximum (1RM) in the full squat and chest press exercises. A total of 96 young women and 256 young men (recreational athletes) performed an incremental test (50–60–70–80% 1RM) comprising the bench press and full squat exercises in two different sessions. The individual load and velocity ratios were established through the MPV. Data were analyzed using SPSS software version 25.0, with the significance level set at 5%. The following findings were revealed: highly linear load-velocity relationships in the group of women (r = 0.806 in the squat, and r = 0.872 in the bench press) and in the group of men (r = 0.832 and r = 0.880, respectively); significant differences (p < 0.001) in the MPV at 50–70–80% 1RM between the bench press and the full squat in men and at 70–80% 1RM in women; and a high variability in the MPV (11.49% to 22.63) in the bench press and full squat (11.58% to 25.15%) was observed in women and men (11.31% to 21.06%, and 9.26% to 24.2%) at the different percentages of 1RM evaluated. These results suggest that the load-velocity ratio in non-strength-trained subjects should be determined individually to more precisely establish the relative load to be used in a full squat and bench press training program

SM-COLSARSPROT: Highly Immunogenic Supramutational Synthetic Peptides Covering the World’s Population

Patarroyo, Manuel-Alfonso | 2022

Fifty ~20–amino acid (aa)–long peptides were selected from functionally relevant SARS-CoV-2 S, M, and E proteins for trial B-21 and another 53 common ones, plus some new ones derived from the virus’ main genetic variants for complementary trial C-21. Peptide selection was based on tremendous SARS-CoV-2 genetic variability for analysing them concerning vast human immunogenetic polymorphism for developing the first supramutational, Colombian SARS-protection (SM-COLSARSPROT), peptide mixture. Specific physicochemical rules were followed, i.e., aa predilection for polyproline type II left-handed (PPIIL) formation, replacing β-branched, aromatic aa, short-chain backbone H-bond-forming residues, π-π interactions (n→π* and π-CH), aa interaction with π systems, and molecular fragments able to interact with them, disrupting PPIIL propensity formation. All these modified structures had PPIIL formation propensity to enable target peptide interaction with human leukocyte antigen-DRβ1* (HLA-DRβ1*) molecules to mediate antigen presentation and induce an appropriate immune response. Such modified peptides were designed for human use; however, they induced high antibody titres against S, M, and E parental mutant peptides and neutralising antibodies when suitably modified and chemically synthesised for immunising 61 major histocompatibility complex class II (MHCII) DNA genotyped Aotus monkeys (matched with their corresponding HLA-DRβ1* molecules), predicted to cover 77.5% to 83.1% of the world’s population. Such chemically synthesised peptide mixture represents an extremely pure, stable, reliable, and cheap vaccine for COVID-19 pandemic control, providing a new approach for a logical, rational, and soundly established methodology for other vaccine development.

Seroprevalence of antibodies to Chlamydia abortus and risk factors in cattle from Villavicencio, Colombia

Orjuela, Agustín Góngora | 2022

Chlamydia abortus is a Gram-negative obligate intracellular bacterium, responsible for abortions and reproductive problems. The disease has a high zoonotic potential and causes great economic losses in ruminant farmers. A cross-sectional study was carried out with 514 cattle from 24 farms of Villavicencio, Colombia. The blood samples were collected from each individual animal and analyzed by Indirect Elisa for immunoglobulin G (IgG) in blood serum (Idexx Chlamydiosis total Ab test). A serum was considered positive when the optical density (OD) of the sample was ≥30% of that of the positive control serum. Data on potential risk factors associated with the disease were collected through a questionnaire in each farm and analyzed. The individual and herd prevalence was estimated. A risk factors analysis was performed through univariate and multivariable using the software SPSS version 20. The animal level seroprevalence was found to be 47.1% and the herd 91.6%. The prevalence in cattle aged 0–1, 1–3 and >4 years was 23.8%; 31.4% and 51.4% respectively. The risk factors associated with the prevalence of disease were female sex (OR = 2.102 CI: 1.066–4.144), age older than 4 years (OR = 2.707 CI: 1.667–4.394), presence of canines on the farm (OR = 2.556 CI: 1.560–4.189) and retention of placenta (OR = 2.678 CI: 1.670–4.295). A high prevalence was identified, suggesting natural infection where the pathogen could be transmitted to humans at the animal-human interface.

Swine as the Animal Model for Testing New Formulations of Anti-Inflammatory Drugs: Carprofen Pharmacokinetics and Bioavailability of the Intramuscular Route

Gómez-Segura, Lídia | 2022

Carprofen (CP) is a non-steroidal anti-inflammatory drug (NSAID) frequently used to treat respiratory diseases in numerous small animals, but also in large species. CP is a formidable candidate for further therapeutic research of human inflammatory diseases using the pig as an animal model. However, CP administration in swine is very uncommon and respective pharmacokinetics/bioavailability studies are scarce. A simultaneous population pharmacokinetic analysis after CP intravenous and intramuscular administrations in pigs has shown high extent and rate of absorption and a similar distribution profile with respect to man and other mammals. However, clearance and half-life values found in swine suggest a slower elimination process than that observed in man and some other animal species. Although not reported in other species, liver and kidney concentrations achieved at 48 h post-intramuscular administration in pigs were ten times lower than those found in plasma. Simulations pointed to 4 mg/kg every 24 h as the best dosage regimen to achieve similar therapeutic levels to those observed in other animal species. All these findings support the use of pig as an animal model to study the anti-inflammatory effects of CP in humans.

Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections

Araujo, Zaida | 2022

Diagnosis and treatment of active tuberculosis (ATB) as well as latent tuberculosis infection (LTBI) are required for efective tuberculosis (TB) control, especially in TB endemic area. The usefulness of conventional tests to distinguish between ATB and LTBI has remained challenging. The present study was aimed to demonstrate the usefulness of the serological response to synthetic peptides from Mycobacterium tuberculosis (Mtb) antigens for discrimination between ATB and LTBI in Warao Amerindians. Serum IgG antibody levels were measured by the indirect ELISA assay using 22 designed and synthesized peptides derived from immunogenic Mtb ESAT-6 and Ag85A proteins. A total of 211 adult Warao Amerindians were included; cases with active TB (ATB, n=75), latent TB infection (LTBI, n=85) and non-infected (NI, n=51). The approach’s diagnostic information was compared using receiver operating characteristic (ROC) curves. For ATB diagnostic performance between ATB and NI; ESAT-6; P-12037 had 100% of sensitivity (AUC=0.812; 0.733 to 0.891 95% CI); and Ag85A; P-10997 had 100% of specifcity (AUC=0.691; 0.597 to 0.785 95% CI); and ATB and LTBI; Ag85A; P-29878 had 100% of sensitivity (AUC=0.741; 0.666–0.817 95% CI), and P-29879 had 99% of specifcity (AUC=0.679; 0.593–0.765 95% CI). While that ESAT-6 P-12037 also allowed diferentiation between LTBI and NI or healthy ones. It had 98.8% of sensitivity and 98.0% of specifcity (AUC=0.640; 0.545–0.735 95% CI). The potential of combination-antigen immunoassays with peptides could discriminate between Warao Amerindians with ATB, LTBI and NI. Further validation of this approach could lead to developing a complementary tool for rapid diagnosis of TB infections.

Descripción morfológica y caracterización molecular de los hongos asociados a la raíz de Masdevallia coccinea Linden ex Lindl

Triana Vallejos, Jairo Alberto | 2022

Masdevallia coccinea es una orquídea llamativa, endémica de Colombia, empleada en la generación de híbridos ornamentales con más de una década, clasificada como una especie en peligro de extinción. Entre las técnicas usadas para la propagación in vitro y ex situ de especies de la familia Orchidaceae, se estudia la simbiosis micorrízica, debido a que esta familia micoheterótrofa depende de una correlación con estos hongos para subsistir en las primeras etapas de desarrollo en estado silvestre. Con el objetivo de caracterizar e identificar los hongos asociados a las raíces de M. coccinea, se realizó un estudio histológico en raíces y, a partir de micropreparados, se caracterizó morfológicamente micro, macroscópica y molecularmente diez aislamientos. Se identificó a M. coccinea como una orquídea que presenta diferentes patrones de colonización micorrízicos y con posibles efectos endófitos de los géneros Aspergillus, Scopulariopsis, Trichoderma, Ilyonectria y del orden Xylariales en condiciones ex situ

Nonstationary extreme precipitation in Brazil

Cortez, Bianca | 2022

This study provides the first estimates of the frequency distribution of observational and future maximum daily precipitation considering nonstationarity for the entire Brazilian territory. We assess observational data from 1980 to 2015 and projected data from 2020 to 2099 for two climate change scenarios and four downscaled climate models. We modelled extreme precipitation according to the extreme values theory and calculated the precipitation intensity associated with the return periods of 5, 10, 25, 50, and 100 years in nonstationary conditions. The results indicate that nonstationarity is identified in 17.5% of the study area during 1980–2015. The analysis of future climate projections indicated an increase in the return levels of extreme precipitation compared to the historical period in at least 90% of the national territory. It shows that engineering design must urgently consider the nonstationarity of extreme precipitation under the risk of increasingly unsafe infrastructure.

Gobernanza y legitimidad en las federaciones deportivas colombianas

Acosta, José Ramos | 2022

Con base en la investigación que adelantó la Universidad de Ciencias Aplicadas y Ambientales y el Comité Olímpico Colombiano, se elaboró el presente artículo, en el que se determina el estado de la gobernanza y legitimidad de las federaciones deportivas colombianas. Los resultados evidencian la existencia de debilidades relacionadas con el desequilibrio de género, como también, sobre la solidaridad con atletas e instituciones de menor jerarquía; el estudio estableció la necesidad de estructurar la creación de un observatorio de buena gobernanza. La muestra estuvo determinada, por ocho federaciones deportivas colombianas, en una población total de 19, cuya participación está reflejada en dar a conocer a través de elementos puntuales como su página web, sus estatutos, sus planes de gobierno, entre otros, acciones indirectas que evidencian elementos de transparencia, democracia, separación de poderes y solidaridad, los cuales son fundamento de la buena gobernanza y la legitimidad. La investigación se centró en evaluar las federaciones deportivas nacionales, con base en un estudio adelantado por la organización internacional Play the Game que evalúo el estado de la gobernanza y la legitimidad de las federaciones deportivas internacionales, las cuales hacen parte del programa olímpico.

Reconstruction of the origin and dispersal of the worldwide dominant Hepatitis B Virus subgenotype D1

Sequeira Trovão, Nídia | 2022

Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus (HBV). HBV-D1 is the dominant subgenotype in the Mediterranean basin, Eastern Europe, and Asia. However, little is currently known about its evolutionary history and spatio-temporal dynamics. We use Bayesian phylodynamic inference to investigate the temporal history of HBV-D1, for which we calibrate the molecular clock using ancient sequences, and reconstruct the viral global spatial dynamics based, for the first time, on full-length publicly available HBV-D1 genomes from a wide range of sampling dates. We pinpoint the origin of HBV subgenotype D1 before the current era (BCE) in Turkey/Anatolia. The spatial reconstructions reveal global viral transmission with a high degree of mixing. By combining modern-day and ancient sequences, we ensure sufficient temporal signal in HBV-D1 data to enable Bayesian phylodynamic inference using a molecular clock for time calibration. Our results shed light on the worldwide HBV-D1 epidemics and suggest that this originally Middle Eastern virus significantly affects more distant countries, such as those in mainland Europe.

Restauración ecológica participativa y servicios ecosistémicos culturales: una relación necesaria

González-Molina, H. Zolangie | 2022

Antecedentes y Objetivos: La incorporación de los servicios ecosistémicos culturales en los proyectos de restauración ecológica participativa es muy escasa, a pesar de reconocerse como importante. El objetivo de este estudio fue analizar el papel de los servicios ecosistémicos culturales asociados a procesos de restauración ecológica participativa en el Ecoparque Sabana en el municipio Tocancipá, Colombia, mediante la valoración de percepciones y representaciones sociales de los actores del proceso. Métodos: Para valorar las percepciones y representaciones sociales, se aplicó una entrevista semiestructurada a administrativos, trabajadores y visitantes del Ecoparque, así como a habitantes del municipio Tocancipá. Se evaluaron los servicios ecosistémicos de belleza escénica, inspiración espiritual y artística, sentido de lugar, relaciones sociales, ecoturismo, valor educativo y patrimonio cultural. La información se analizó mediante minería de texto, análisis del discurso y análisis de correspondencia múltiple. Resultados clave: Los servicios ecosistémicos culturales cumplen un papel clave dentro del proceso de restauración ecológica participativa. Este tipo de procesos de restauración generaron en la mayoría de los entrevistados (91%) una percepción positiva. Adicionalmente, se encontraron diferencias en las percepciones sobre los servicios ecosistémicos de acuerdo con el grupo de actores al que pertenecen, género y residencia (rural/urbana). El aumento del número de visitas resultó en un incremento del arraigo de los visitantes hacia el proceso de restauración. El valor educativo y el ecoturismo brindan un valor agregado a los procesos de restauración, ya que promueven las relaciones sociales, la conservación y generan relaciones de arraigo. Conclusiones: Incorporar la valoración sociocultural de servicios ecosistémicos, incluyendo los culturales, en los procesos de restauración ecológica participativa es necesario. Se comprobó el fortalecimiento del vínculo entre la recuperación de aspectos biofísicos con los aspectos culturales en la generación de tejido social a medida que avanza la restauración.

Leptospirosis, bovine viral diarrhea and infectious bovine rhinotracheitis: prevalence in Colombian cattle and buffaloes

Arias García, Alejandra | 2022

One of the limiting factors of productive efficiency in cattle and buffalo herds is related to the high prevalence of infectious diseases which affect reproduction. The aim of this study was to determine the prevalence of anti-Leptospira antibodies for bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BoHV-1) in bovine and buffalo herds in Colombia. Blood serum samples were collected from 1100 buffaloes and 1000 cattle. The ELISA technique was used to detect antibodies against BVDV and BoHV-1, and the microscopic agglutination technique to detect anti-Leptospira antibodies. The prevalence of anti-Leptospira antibodies and of BVDV and BoHV-1 in bovine samples was observed in 16, 39.7, and 65% of animals, respectively, while the positivity in samples for the same antibodies in buffalos was detected in 18.7, 27.5 and 51.5%, respectively. Exposure of cattle and buffaloes to BoHV-1 was positively associated with age, higher prevalence rates were observed in older ages. Seropositivity of cattle for BVDV and BoHV-1 was higher in male animals. Activities such as embryo transfer, milking, and needle reuses, as well as the presence of cats and rodents are factors which favor positivity of the herd for BVDV and BoHV-1.