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Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix

Torrenegra, Rubén Darío; Rodríguez, J.; Rodríguez Aguirre, Oscar E.; Palau, Victoria E.; Méndez, Gina M. (2016)

The flavone 3,5,7-trihydroxy-6-methoxyflavone was isolated from leaf extracts of Chromolaena leivensis (Hieron), commonly named plant of cancer. It was identified based on their physicochemical propierties and spectroscopic ...
Antiproliferative activity of extracts of Gnaphalium Gracile H.B.K. against cancer cell lines

Torrenegra Guerrero, Rubén Darío; Rodriguez Mayusa, J.; Mendez Callejas, G.M.; Canter, R.; Whitted, C.; Palau, V.E. (2018)

Ethanol and n-hexane extracts obtained from the leaves and inflorescences of Gnaphalium gracile, were tested at different concentrations to evaluate their antineoplastic activities on pancreatic, colon, and prostate cancer ...
Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

Whitted, Crystal L.; Palau, Victoria E.; Torrenegra, Rubén D.; Rodrigez, Oscar E.; Harirforoosh, Sam (2017)

Background: Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8-trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell ...

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Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix
...
Torrenegra, Rubén Darío | 2016

The flavone 3,5,7-trihydroxy-6-methoxyflavone was isolated from leaf extracts of Chromolaena leivensis (Hieron), commonly named plant of cancer. It was identified based on their physicochemical propierties and spectroscopic data. This compound presented a methoxylation at C6, this is uncommon in flavonoids and which may confer some specificity of its biological activity. The cytotoxic activity of this flavonoid was determined on PC3 (prostate), MDA- MB-231 (breast), HT29 (colon), SiHa (cervix) and A549 (lung) cancer cells, using MTT assay, to assess if the flavonoid contributes to the anticancer activity previously proposed for Chromolaena leivensis. The cytotoxic activity of the 3,5,7-trihydroxy-6- methoxy flavone was similar to that obtained for the flavonoid quercetin but was low compared with the positive control vincristine sulphate. The better value of the inhibitory concentration of fifty percent (IC50) 150 μ M, was achieved on SiHa cell line, while the lower activity: 4008 μ M, was obtained on HT29 cancer cell line. However, severe morphological changes were detected on cytoskeleton and nucleus of the SiHa cells detected by immunofluorescence microscopy analysis of cells exposed to the half of the IC50 concentration obtained for the flavonoid. Data indicate that the flavonoid contributes to the anticancer activity of the extracts of leaves from Chromolaena leivensis, and could broadening the spectrum of flavonoids activity against various types of cancer non hormone-dependent.

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Antiproliferative activity of extracts of Gnaphalium Gracile H.B.K. against cancer cell lines
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Torrenegra Guerrero, Rubén Darío | 2018

Ethanol and n-hexane extracts obtained from the leaves and inflorescences of Gnaphalium gracile, were tested at different concentrations to evaluate their antineoplastic activities on pancreatic, colon, and prostate cancer cell lines by examining mitochondrial function. The polar extracts of both, leaves and inflorescences which contain gnaphalin, quercetin, and 3-methoxy quercetin, exhibited cytotoxicity against every cell line tested with EC50 values ranging between 20.23±1.185 µg/mL and 70.71±1.1419 µg/mL. The most remarkable values were observed in pancreatic cancer Panc 28 and androgen-dependent prostate LnCaP cells, with EC50 values of 20.23±1.185 and ˂25µg/mL, and androgen-independent prostate cancer PC-3, colon HCT-116 and pancreatic MIA PaCa cells with values ranging between 28.84±1.1766 and 34.41±1.057 µg/mL. The non-polar extract derived from leaves demonstrated significant cytotoxicity towards colon cancer HCT-116 cells, with an EC50 of 39.46±1.0617 µg/mL. However, the non-polar extract from the inflorescences did not have an appreciable effect on cell proliferation of any of the cell lines tested except for androgen-independent prostate cancer PC-3 cells with an EC50 of 62.05±1.237 µg/mL. The data obtained support the traditional use of G. gracile and suggest the polar extracts from aerial parts, as an interesting source for the development of novel antineoplastic agents.

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Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography
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Whitted, Crystal L. | 2017

Background: Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8-trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell lines (Thomas et al. in PLoS ONE 7:e39806, 5). Here, we present modified methods for the extraction and quantification of flavones A and B in rat colon tissue after intravenous dosing via high performance liquid chromatography, from the originally described procedure for extraction and quantification in rat plasma (Whitted et al. in J Chromatogr B Analyt Technol Biomed Life Sci 1001:150-155, 7). Results: Modifications included tissue homogenization (1 g tissue: 2 mL water), filtration of the supernatant with a PVDF membrane, and the use of only one calibration curve to determine the concentration of each flavone in colon tissue. Good separation was achieved and representative equations were linear with r 2 ≥ 0.99 for both flavones. Precision and accuracy for flavone A ranged from 0.88-24.03 and 109-116%. Precision and accuracy for flavone B ranged from 1.62-33.56 and 98-113%. Concentrations of 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g of flavone B were detected in rat colon tissue 6 h post dosing. Conclusions: Modifications to the extraction methods for flavone A and flavone B from rat colon tissue had good separation, precision, and accuracy

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Browsing CCB. Artículos indexados en Scopus by Subject "Aceites Volátiles"

Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix ﻿

Antiproliferative activity of extracts of Gnaphalium Gracile H.B.K. against cancer cell lines ﻿

Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography ﻿

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Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix

Antiproliferative activity of extracts of Gnaphalium Gracile H.B.K. against cancer cell lines

Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

Antiproliferative activity of 3,5,7- trihydroxy -6- methoxy flavone obtained from chromolaena leivensis (Hieron) on cancer cell lines of breast, prostate, lung, colon and cervix

Antiproliferative activity of extracts of Gnaphalium Gracile H.B.K. against cancer cell lines

Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography