CCB. Artículos indexados en Scopus
https://repository.udca.edu.co/handle/11158/1830
2024-03-29T06:22:29ZEffect of carbohydrates on the adhesion of Bordetella bronchiseptica to the respiratory epithelium in rabbits
https://repository.udca.edu.co/handle/11158/5625
Effect of carbohydrates on the adhesion of Bordetella bronchiseptica to the respiratory epithelium in rabbits
Patiño, Pilar; Gallego, Carolina; Martínez, Nhora María; Iregui, Carlos Arturo; Rey, Alba
This study proposes an ecological approach for preventing respiratory tract infections caused by Bordetella bronchiseptica in mammals using a mixture of carbohydrates. In an in vivo study, 51-day-old New Zealand rabbits were treated with a solution containing 1 × 107 CFUs of B. bronchiseptica and 250 μg of one of the following carbohydrates: N acetylglucosamine (GlcNAc), N acetylgalactosamine (GalNAc), alpha methyl mannose (AmeMan), alpha methyl glucose (AmeGlc) and sialic acid (Neu5AC). Positive (B. bronchiseptica) and negative (Physiological Saline Solution (PSS)) controls were included. Animals treated with GlcNAc or AmeGlc showed no clinical signs of infection and exhibited a significant reduction (p < 0.05) in the severity of microscopic lesions evaluated in the nasal cavity and lung compared with the positive controls. Additionally, the presence of bacteria was not detected through microbiological isolation or PCR in the lungs of animals treated with these sugars. Use of a mixture of GlcNAc and AmeGlc resulted in greater inhibition of microscopic lesions, with a significant reduction (p < 0.05) in the severity of these lesions compared to the results obtained using individual sugars. Furthermore, the bacterium was not detected through microbiological isolation, Polymerase Chain Reaction (PCR) or indirect immunoperoxidase (IIP) in this group.
2024-01-01T00:00:00ZUnderstanding the sociocultural valuation of ecosystem services in urban parks: a Colombian study case
https://repository.udca.edu.co/handle/11158/5623
Understanding the sociocultural valuation of ecosystem services in urban parks: a Colombian study case
Granobles Velandia, Fabian Andres; Trilleras, Jenny M.; Romero-Duque, Luz Piedad; Quijas, Sandra
The significance of urban ecosystem services on the perception of parks among the public is profound. Parks are valued for their functional benefits, the experiences they provide, and their relationship with green spaces. To better understand the sociocultural values associated with five urban parks in Tunja (Boyacá, Colombia), in this case study, we interviewed three types of stakeholders: decision-makers, social leaders, and park users. We employed semi-structured interviews to identify the perception and orientation values of different stakeholders. According to the results, stakeholders recognize 18 ecosystem services as the most important, with cultural ecosystem services having the highest perception value. We found that socio-cultural values are influenced more by the characteristics of the parks than their size, and socio-demographic aspects of stakeholders such as age, level of education, and gender had no significant effect. We conclude that people value urban parks for the benefits associated with trees and the emotional connections they develop with them over time, assigning intangible value to city parks. However, we also identified discrepancies in values between governmental and non-governmental stakeholders that may adversely affect decision-making and policy formulation. This information can be valuable to urban planners who seek to assess and integrate measures that promote green spaces in cities to achieve sustainability
2024-01-01T00:00:00ZBabesia bovis RON2 binds to bovine erythrocytes through a highly conserved epitope
https://repository.udca.edu.co/handle/11158/5622
Babesia bovis RON2 binds to bovine erythrocytes through a highly conserved epitope
Cuy Chaparro, Laura; Barney, Danny; Arevalo-Pinzon, Gabriela; Reyes Santofimio, César Mauricio; Moreno-Perez, Darwin Andres; Patarroyo, Manuel-Alfonso
B. bovis invasion of bovine erythrocytes requires tight junction formation involving AMA-1/RON2 complex
interaction. RON2 has been considered a vaccine candidate since antibodies targeting the protein can inhibit
parasite invasion of target cells; however, the mechanism controlling B. bovis RON2 interaction with red blood
cells is not yet fully understood. This study was thus aimed at identifying B. bovis RON2 protein regions associated with interaction with bovine erythrocytes. Natural selection analysis of the ron2 gene identified predominantly negative selection signals in the C-terminal region. Interestingly, protein-cell and competition assays
highlighted the RON2-C region’s role in peptide 42918-mediated erythrocyte binding, probably to a sialoglycoprotein receptor. This peptide (1218SFIMVKPPALHCVLKPVETL1237) lies within an intrinsically disordered region of the RON2 secondary structure flanked by two helical residues. The study provides, for the first time, valuable insights into RON2’s role in interaction with its target cells. Future studies are required for studying the peptide’s potential as an anti-B. bovis vaccine component
2024-01-01T00:00:00ZCryopreserved semen of Piaractus orinoquensis (Serrasalmidae): post-thaw storage times and activating solutions
https://repository.udca.edu.co/handle/11158/5617
Cryopreserved semen of Piaractus orinoquensis (Serrasalmidae): post-thaw storage times and activating solutions
Medina Robles, Victor Mauricio; Suárez-Martínez, Roger Oswaldo; Baldisserotto, Bernardo; Cruz Casallas, Pablo Emilio
Piaractus orinoquensis is a native species from the Colombian Orinoquia and several studies have contributed to the standardization of protocols for seminal cryopreservation of this species. The main goal of this study was to evaluate the sperm motility, duration of sperm motility (DM), pH, sperm membrane integrity (SMI) and fertility of semen cryopreserved for seven years and subjected to different post-thaw storage times (PST). Semen from five males (3.04 ± 0.3 kg) was diluted 1:4 (semen:diluent) with 10 % dimethylsulfoxide, 5.5 % glucose and 12 % egg yolk in 4 mL macrotubes and frozen in liquid nitrogen. Four PST were evaluated: 0, 15, 45 and 60 min. The sperm cells were activated with 1 % NaHCO3 (SB) and 0.9 % NaCl (SC) to determine sperm motility, DM, fertility rate, SMI and pH through PST. Significative motility reduction (p < .05) was observed through the PST and between the PST of 0 and 60 min for DM, both for treatments activated with SB and with SC. The SMI had a significant reduction (p < .05) after 60 min of post-thaw storage and the pH did not vary during PST. The fertility rate decreased significantly (p < .05) between time 0 and 60 min of PST. PST affects the seminal quality and fertility of P. orinoquensis, the best fertilization results were obtained by activating post-thawing sperm motility with 1 % SB. The long-term cryopreserved semen (seven years) of the species maintains its fertilization capacity with values like those obtained with fresh sperm; Piaractus orinoquensis es una especie de la Orinoquía Colombiana en la cual se han realizado numerosos estudios para estandarizar protocolos de crioconservación seminal. El objeto de este estudio fue evaluar la motilidad espermática, duración de la motilidad espermática (DM), pH, integridad de membrana espermática (SMI) y fertilidad de semen crioconservado por siete años, sometido a diferentes tiempos de almacenamiento posdescongelación (PST). Semen de 5 machos (3,4 ± 0,3 kg)fue diluido 1:4 (semen: diluyente) con dimetilsulfóxido 10 %, glucosa 5,5 %, yema de huevo 12 %, en macrotubos de 4 mL y congelado en nitrógeno líquido. Cuatro PST fueron evaluados: 0, 15, 45 y 60 min. Las células espermáticas fueron activadas con NaHCO3 1% (SB) y NaCl 0,9 % (SC) para determinar motilidad espermática, DM, tasa de fertilización, SMI y pH a través de PST. Se observó reducción significativa de motilidad (p < ,05) a través del PST, también en DM entre tiempo 0 y tiempo 60 min. de PST en semen activado con SB y SC. SMI tuvo una reducción significativa (p < ,05) luego de 60 min. de almacenamiento posdescongelación y el pH no varió durante el PST. La tasa de fertilización decayó significativamente (p < ,05) entre 0 y 60 minutos de PST. La calidad seminal y fertilidad en P. orinoquensisfue afectada por PST. Los mejores resultados de fertilización se obtuvieron activando la motilidad con SB. El semen crioconservado a largo plazo (siete años), conservó su capacidad fertilizante con valores similares a los obtenidos con semen fresco
2023-01-01T00:00:00Z