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Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical

Ortiz Trujillo, Bibiana; Ospino, Benjamín; Trujillo, Miosotis (Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2007, 2007-07)

This research determined the possibility to change the hydroxyethyl starch (HES), conventional crioprotector, for two sorts of mandioca starches made by national industry. The study verified the influence of the mandioca ...
Evaluación de la movilidad del semen criopreservado de caballos criollo colombiano por un sistema analizador de clase

Restrepo, Giovanni; Ocampo, Daniel; Velásquez, Andrés (Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2013, 2013-07)

La reproducción juega un papel fundamental en el mejoramiento genético de los equinos. La criopreservación del semen equino es una técnica complementaria a los procesos de biotecnología reproductiva; sin embargo, solamente ...
Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification

Bernal Ulloa, Sandra Milena; Lucas Hahn, Andrea; Herrmann, Doris; Hadeler, Klaus; Aldag, Patrick (2016)

Cryopreservation of in vitro produced bovine embryos is associated with significantly reduced survival rates, mainly due to insufficient quality of the embryos. Caffeine supplementation during IVM has been used to delay ...
Vitrificación de embriones bovinos producidos in vitro

Ramírez, Orlando; Bernal, Sandra (Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2012, 2012-07)

Around the world and in Colombia the in vitro cattle embryo production has increased in the last years. The embryo transfer of in vitro produced embryos is mainly realized with fresh embryos, due to the ...
Vitrificación de embriones bovinos producidos in vitro

Garzón Niño, Lina María (Facultad de Ciencias AgropecuariasMedicina Veterinaria, 2011)

Esta investigación suministra información sobre la criopreservación de embriones producidos in vitro tanto a los estudiantes como a los profesionales del área de las ciencias pecuarias, para que obtengan mejores resultados ...

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Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical
...
Ortiz Trujillo, Bibiana | 2007-07

This research determined the possibility to change the hydroxyethyl starch (HES), conventional crioprotector, for two sorts of mandioca starches made by national industry. The study verified the influence of the mandioca starches on the hidrogenions amount in blood and confirmed the estability of starches in umbilical cord blood envelop and established its molecular stability inside a medium with amylases enzymes and mononuclear cells such as the umbilical cordon blood. The mononuclear cells viability six months after being criopreservated with each starch at –80ºC, using HES as a reference or standard, was also assessed. Results showed that the umbilical cord blood, outside the organism for 30 minutes is more affected by the mandioca starches than by HES. In spite of the similar structure of the mandioca starch and HES, the latter one is more resistant in the blood environment to the enzymatic attack of amylases because of its modified structure, therefore it is more resistance to withstand the blood conditions. It was corroborated that HES was able to keep the mononuclear cell viability over 70%, but mandioca starches did not, because of their influence on the pH which became lethal to cells. It was concluded, that the amilopectine of mandioca starches with some modifications in its molecular structure such as hydroxyethylation could work as a crioprotector for mononuclear blood cells, provided that the pH is controlled.

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Evaluación de la movilidad del semen criopreservado de caballos criollo colombiano por un sistema analizador de clase
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Restrepo, Giovanni | 2013-07

La reproducción juega un papel fundamental en el mejoramiento genético de los equinos. La criopreservación del semen equino es una técnica complementaria a los procesos de biotecnología reproductiva; sin embargo, solamente el 40% de los caballos producen semen que se puede criopreservar, con una alta variabilidad en su fertilidad. La confiabilidad de los métodos de evaluación del semen es trascendente para determinar su fertilidad potencial; no obstante, el análisis convencional por evaluación microscópica ha sido poco efectivo, en este sentido. El análisis de semen asistido por computador (CASA) permite una medición objetiva de la movilidad de semen. Esta investigación tuvo como objetivo comparar la evaluación de la movilidad del semen equino, mediante los métodos convencional y SCA® (CASA). El semen de tres caballos de la raza criollo colombiano (tres eyaculados cada uno) fue congelado, en un diluyente (4% de yema de huevo, 5% de dimetilformamida). La movilidad del semen descongelado, se determinó de forma convencional por microscopia de contraste de fase y mediante el software SCA® . Para el análisis estadístico, se ajustó un modelo lineal generalizado (GLM) y se determinó la asociación entre las variables, a través de análisis de correlación y regresión. Se hallaron promedios de movilidad total de 54,3 ± 10,1 y 61,8 ± 13,9, para evaluación convencional y por el sistema SCA® , respectivamente. No se encontró diferencia significativa entre los métodos de análisis. Se concluye que el análisis de semen equino, mediante el método convencional y el sistema SCA® , es equiparable, para analizar la movilidad del semen equino criopreservado.

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Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification
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Bernal Ulloa, Sandra Milena | 2016

Cryopreservation of in vitro produced bovine embryos is associated with significantly reduced survival rates, mainly due to insufficient quality of the embryos. Caffeine supplementation during IVM has been used to delay meiotic resumption and concomitantly also increased embryo quality. Here, we investigated the influence of pre-IVM with caffeine on oocyte maturation, intraoocyte cAMP concentration, developmental competence after IVF, and blastocyst cryotolerance. Oocytes were obtained by slicing of ovaries and were submitted to either 2 hours culture before IVM with or without caffeine (0, 1, 5, 10, 20, 30 mM), or standard IVM (no pre-IVM). Oocytes were in vitro matured and fertilized and zygotes were cultured under standard in vitro conditions until Day 8. Expanded blastocysts derived from either standard control or the 10-mM caffeine treatments were submitted to vitrification. Caffeine delayed meiotic resumption after 9-hour IVM in a concentration-dependent manner. The cAMP levels were similar before and after IVM. Matured oocytes, cleavage, and blastocyst rates were reduced in the 30-mM caffeine concentration and were similar among the other treatment groups. Number and proportion of inner cell mass and trophectoderm cells in blastocysts did not differ among treatments. Forty-eight hours after thawing, hatching rates were higher in the 10-mM caffeine group (73.8%) compared with the standard control (59.7%). Reexpansion rates and total number of cells after 48 hours were similar in both treatments. The ratio of live/total cells was higher in the caffeine treatment. These results suggest that caffeine supplementation before IVM delayed meiotic resumption and improved blastocyst quality shown in higher cryotolerance.

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Vitrificación de embriones bovinos producidos in vitro
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Ramírez, Orlando | 2012-07

Around the world and in Colombia the in vitro cattle embryo production has increased in the last years. The embryo transfer of in vitro produced embryos is mainly realized with fresh embryos, due to the possible injuries induced during the cryopreservation process. Vitrification is a tool applied in assisted reproductive technologies (ARTs) and it is used as an alternative for germplasm cryopreservation (sperm, oocytes and embryos) in human and veterinary medicine. Despite major efforts focused on the development of the technique, its commercial application is scarce in the embryo production industry and it is employed mainly in research. In this review, different aspects involved in the in vitro embryo vitrification technique, such as protocols, main advantages, limitations and some results, are discussed.

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Vitrificación de embriones bovinos producidos in vitro
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Garzón Niño, Lina María | 2011

Esta investigación suministra información sobre la criopreservación de embriones producidos in vitro tanto a los estudiantes como a los profesionales del área de las ciencias pecuarias, para que obtengan mejores resultados y disminuyan las pérdidas económicas para la implementación de la técnica en las empresas que comercializan material genético bovino.

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Browsing by Subject "Criopreservación"

Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical ﻿

Evaluación de la movilidad del semen criopreservado de caballos criollo colombiano por un sistema analizador de clase ﻿

Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification ﻿

Vitrificación de embriones bovinos producidos in vitro ﻿

Vitrificación de embriones bovinos producidos in vitro ﻿

Envíos recientes

Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical

Evaluación de la movilidad del semen criopreservado de caballos criollo colombiano por un sistema analizador de clase

Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification

Vitrificación de embriones bovinos producidos in vitro

Vitrificación de embriones bovinos producidos in vitro

Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical

Evaluación de la movilidad del semen criopreservado de caballos criollo colombiano por un sistema analizador de clase

Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification

Vitrificación de embriones bovinos producidos in vitro

Vitrificación de embriones bovinos producidos in vitro