Envíos recientes

Application of proteomic to investigate the different degrees of meat tenderness in Nellore breed

Malheiros, J. M. | 2021

This study describes the association between meat tenderness and abundance of soluble muscle proteins in Nellore bulls (Bos indicus) using a proteomic approach. We evaluated shear force (SF) of Longissimus thoracis muscle 24 h after slaughter and selected three experimental groups of animals with moderately tender (TE; SF = 3.9 ± 0.7 kg), moderately tough (TO; SF = 5.6 ± 0.7 kg) and very tough meat (TO+; SF = 7.9 ± 1.4 kg). Proteome was investigated by two-dimensional electrophoresis (2D-PAGE) in combination with electrospray ionization-tandem mass spectrometry (ESI–MS/MS). The metabolic proteins triosephosphate isomerase (TPI1) and phosphoglucomutase 1 (PGM1), the structural protein profilin 1 (PFN1), and cytosol aminopeptidase (LAP3) were up-regulated (P < 0.05) in the TE meat group when compared to the TO and TO+ groups. Actin structural proteins (ACTA1, ACTB, and ACTG1), the oxidative stress protein peroxiredoxin (PRDX6, PRDX2, PRDX1, and PARK7), heat shock protein isoforms, and co-chaperones (CDC37 and STIP1) were up-regulated (P < 0.05) in the TO and TO+ meat groups. In addition, we also identified proteins PFN1, LAP3, PRDX1, PRDX2, HSPD1, and ARHGDIA to be associated with beef tenderness. The results reported herein demonstrated that meat tenderness in Nellore cattle depends on the modulation and expression of a set of proteins involved in different biological pathways.

Biología molecular e inmunología veterinaria: Informe de actividades 2019-II

Avendaño Valenzuela, Catalina | 2020-01-13

Biología molecular e inmunología veterinaria: Informe de actividades 2021-I

Avendaño Valenzuela, Catalina | 2021-06-23

Se realizó la socialización de un artículo, reunión formal y los resultados de avance de uno de los proyectos de grado que se vienen ejecutando en este momento.

Biotecnología Agrícola: Informe de actividades 2018-II

Bailón, Concepción | 2018-12

1. Trabajos de grado (pregrado) • Aislamiento e Identificación de micorrizas presentes en la orquídea., Masdevallia coccinea, y germinación simbiótica de semillas de orquídea. • Propagación in vitro de agraz.

Estados hipertensivos en el embarazo: revisión

Beltrán Chaparro, Lina Vanessa | 2014-07

La enfermedad hipertensiva en el embarazo es una de las primeras causas de morbimortalidad. La siguiente revisión pretende dar una visión de su fisiopatología, a la luz de la bio-logía molecular. Se analizan los factores de riesgo, basados en la evidencia, el diagnóstico y el manejo, según las guías nacionales e internacionales, que permite tener un enfoque actualizado de una patología frecuente, para el especialista y para el médico general. Los autores desean que este docu-mento sea de utilidad en la práctica médica.

Semillero de Investigación ORIATROS - Bioquímica clínica y genética toxicológica: Informe de actividades 2019-I

Vergara García, Angela Patricia | 2019-06-26

Técnica de extracción de adn a partir de la glándula de seda del gusano de seda bombyx morí (lepidoptera: bombycidae)

Mejía Cruz, Rodolfo Alberto | 2006-01

Selection for production of puré lines of the silkworm (Bombyx morí), starting with commercial hybrids, is a long and expensive process, however, molecular biology techniques can shorten the time and allow to determine favorable characteristics for silk production. The objective of this work consisted in establishing a protocol for the extraction of DNA of good quality and quantity of the silkworm. The research was carried out at the molecular biology laboratory of the Ü.D.C.A at Bogotá, using larvae coming from the breeds of segregant lines maintained in the laboratory. For the extraction of DMA the posterior, médium and anterior part, placed in a Buffer extraction solution with Tris HCI, MaCI and EDTA pH 8, of the silk gland was used. Subsequently the samples were washed with phenol chloroform and isoamylic chloroform. For the DMA precipitation frozen ethanol was applied. Electrophoresis in agarosa was performed and quantified in a mini-fluorometer with a fixed longitude wave, obtaining a range between 0.21/xg/ml and 1.5/xg/ml of DMA. The quality and quantity of the DMA of the posterior part of the silk gland of five day oíd larvae of the last instar was superior to that obtained from larvae of other ages and other segments of the gland. Therefore the posterior part of the silk gland is considered to be the most adequate tissue for the DMA extraction process related to molecular analysis of silk productivity components.