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ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis

Araujo, Zaida; Fernández de Larrea, Carlos; López, Diana; Isern-Kebschull, Jaime; de Waard, Jacobus H.; Hagel, Isabel; Camargo, Milena; Vanegas, Magnolia ; Patarroyo, Manuel-Alfonso (2021)

Background. Tuberculosis (TB) is being underdetected in children as most are smear-negative. This work was aimed at evaluating ESAT-6 and Ag85A synthetic peptides’ serodiagnostic potential for diagnosing children having a ...
Stereo electronic principles for selecting fully-protective, chemically-synthesised malaria vaccines

Patarroyo, Manuel-Elkin; Bermudez, Adriana; Alba, Martha patricia; Patarroyo, Manuel-Alfonso; Suárez, Carlos; Aza-Conde, Jorge; Moreno-Vranich, Armando; Vanegas, Magnolia (2022)

Major histocompatibility class II molecule-peptide-T-cell receptor (MHCII-p-TCR) complex-mediated antigen presentation for a minimal subunit-based, multi-epitope, multistage, chemically-synthesised antimalarial vaccine is ...

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ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis
...
Araujo, Zaida | 2021

Background. Tuberculosis (TB) is being underdetected in children as most are smear-negative. This work was aimed at evaluating ESAT-6 and Ag85A synthetic peptides’ serodiagnostic potential for diagnosing children having a clinical suspicion of TB. Methods. The study involved 438 children: 77 Creole nonindigenous (13 suspected of having TB and 64 healthy ones) and 361 Warao indigenous children (39 suspected of TB and 322 healthy children). The approach’s diagnostic information was compared using operational characteristics and receiver-operating characteristic (ROC) curves. Results. Ag85A P-29879 had 94.6% sensitivity (: 0.651 to 0.819 95% CI) in indigenous children. ESAT-6 P-12036 and P-12037 had 100% and 92.3% of sensitivity (: 0.929: 0.846 to 0.975 95% CI and 0.791: 63.9 to 98.7 95% CI, respectively) in Creole children. ESAT-6 peptides also allowed a differentiation between children with TB and healthy ones. Conclusions. Further validation of this approach could lead to developing a complementary tool for rapid TB diagnosis in children.

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Stereo electronic principles for selecting fully-protective, chemically-synthesised malaria vaccines
...
Patarroyo, Manuel-Elkin | 2022

Major histocompatibility class II molecule-peptide-T-cell receptor (MHCII-p-TCR) complex-mediated antigen presentation for a minimal subunit-based, multi-epitope, multistage, chemically-synthesised antimalarial vaccine is essential for inducing an appropriate immune response. Deep understanding of this MHCII-p-TCR complex’s stereo-electronic characteristics is fundamental for vaccine development. This review encapsulates the main principles for achieving such epitopes’ perfect fit into MHC-II human (HLADRβ̞1*) or Aotus (Aona DR) molecules. The enormous relevance of several amino acids’ physico-chemical characteristics is analysed in-depth, as is data regarding a 26.5 ± 2.5Å distance between the farthest atoms fitting into HLA-DRβ1* structures’ Pockets 1 to 9, the role of polyproline II-like (PPIIL) structures having their O and N backbone atoms orientated for establishing H-bonds with specific HLA-DRβ1*-peptide binding region (PBR) residues. The importance of residues having specific charge and orientation towards the TCR for inducing appropriate immune activation, amino acids’ role and that of structures interfering with PPIIL formation and other principles are demonstrated which have to be taken into account when designing immune, protection-inducing peptide structures (IMPIPS) against diseases scourging humankind, malaria being one of them

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ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis ﻿

Stereo electronic principles for selecting fully-protective, chemically-synthesised malaria vaccines ﻿

Envíos recientes

ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis

Stereo electronic principles for selecting fully-protective, chemically-synthesised malaria vaccines

ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis

Stereo electronic principles for selecting fully-protective, chemically-synthesised malaria vaccines