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Interactome Analysis of Microtubule-targeting Agents Reveals Cytotoxicity Bases in Normal Cells

Gutiérrez Escobar, Andrés Julián; Méndez Callejas, Gina (2017)

Cancer causes millions of deaths annually and microtubule-targeting agents (MTAs) are the most commonly-used anti-cancer drugs. However, the high toxicity of MTAs on normal cells raises great concern. Due to the non-selectivity ...
NMR spectroscopy and antioxidant activity of flavanones and flavones isolated from Chromolaena tacotana (Klatt) R.M. King & H. Rob.

Torrenegra Guerrero, Rubén; Bautista Bautista, L; Rodríguez Mayusa, Jeanet; Méndez Callejas, Gina (2020)

Chromolaena tacotana is considered as a source of flavonoids. Here we examined the content and antioxidant properties of flavones and flavanones from the leaves of the plant. Four flavonoids, including (Cta) 5, 4' ...
Rapid evolution of the Helicobacter pylori AlpA adhesin in a high gastric cancer risk region from Colombia

Gutiérrez Escobar, Andrés Julián; Méndez Callejas, Gina; Acevedo, Orlando; Bravo, Maria Mercedes (2018)

To be able to survive, Helicobacter pylori must adhere to the gastric epithelial cells of its human host. For this purpose, the bacterium employs an array of adhesins, for example, AlpA. The adhesin AlpA has been proposed ...

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Interactome Analysis of Microtubule-targeting Agents Reveals Cytotoxicity Bases in Normal Cells
...
Gutiérrez Escobar, Andrés Julián | 2017

Cancer causes millions of deaths annually and microtubule-targeting agents (MTAs) are the most commonly-used anti-cancer drugs. However, the high toxicity of MTAs on normal cells raises great concern. Due to the non-selectivity of MTA targets, we analyzed the interaction network in a non-cancerous human cell. Subnetworks of fourteen MTAs were reconstructed and the merged network was compared against a randomized network to evaluate the functional richness. We found that 71.4% of the MTA interactome nodes are shared, which affects cellular processes such as apoptosis, cell differentiation, cell cycle control, stress response, and regulation of energy metabolism. Additionally, possible secondary targets were identified as client proteins of interphase microtubules. MTAs affect apoptosis signaling pathways by interacting with client proteins of interphase microtubules, suggesting that their primary targets are non-tumor cells. The paclitaxel and doxorubicin networks share essential topological axes, suggesting synergistic effects. This may explain the exacerbated toxicity observed when paclitaxel and doxorubicin are used in combination for cancer treatment.

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NMR spectroscopy and antioxidant activity of flavanones and flavones isolated from Chromolaena tacotana (Klatt) R.M. King & H. Rob.
...
Torrenegra Guerrero, Rubén | 2020

Chromolaena tacotana is considered as a source of flavonoids. Here we examined the content and antioxidant properties of flavones and flavanones from the leaves of the plant. Four flavonoids, including (Cta) 5, 4' dihydroxy-7-methoxy flavanone, (Ctb) 3,5,3'-trihydroxy-7,4'-dimethoxyflavone; (Ctc), 3,4'-dihydroxy -5,7-dimethoxyflavanone; and (Ctd) 4'-hydroxy-5,7-dimethoxyflavanone, were isolated from leaves extracts, were identified by their NMR spectroscopic data, and then free radical scavenging activities of the flavonoids were assessed against DPPH. The antioxidant activity for the flavanone Ctb was the highest even compared to that of quercetin, with IC50 of 6.27 μg/mL and 8.67 μg/mL respectively. The flavanones Cta, Ctc and Ctd presented a lowest activity against free radicals as expected according to their molecular substituents and the position within the structure. Data obtained from this study support the ethnomedicinal use of the leaves of C. tacotana for an antioxidant purpose.

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Rapid evolution of the Helicobacter pylori AlpA adhesin in a high gastric cancer risk region from Colombia
...
Gutiérrez Escobar, Andrés Julián | 2018

To be able to survive, Helicobacter pylori must adhere to the gastric epithelial cells of its human host. For this purpose, the bacterium employs an array of adhesins, for example, AlpA. The adhesin AlpA has been proposed as a major adhesin because of its critical role in human stomach colonization. Therefore, understanding how AlpA evolved could be important for the development of new diagnostic strategies. However, the genetic variation and microevolutionary patterns of alpA have not been described in Colombia. The study aim was to describe the variation patterns and microevolutionary process of alpA in Colombian clinical isolates of H. pylori. The existing polymorphisms, which are deviations from the neutral model of molecular evolution, and the genetic differentiation of the alpA gene from Colombian clinical isolates of H. pylori were determined. The analysis shows that gene conversion and purifying selection have shaped the evolution of three different variants of alpA in Colombia.

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Browsing by Author "Méndez Callejas, Gina"

Interactome Analysis of Microtubule-targeting Agents Reveals Cytotoxicity Bases in Normal Cells ﻿

NMR spectroscopy and antioxidant activity of flavanones and flavones isolated from Chromolaena tacotana (Klatt) R.M. King & H. Rob. ﻿

Rapid evolution of the Helicobacter pylori AlpA adhesin in a high gastric cancer risk region from Colombia ﻿

Envíos recientes

Interactome Analysis of Microtubule-targeting Agents Reveals Cytotoxicity Bases in Normal Cells

NMR spectroscopy and antioxidant activity of flavanones and flavones isolated from Chromolaena tacotana (Klatt) R.M. King & H. Rob.

Rapid evolution of the Helicobacter pylori AlpA adhesin in a high gastric cancer risk region from Colombia

Interactome Analysis of Microtubule-targeting Agents Reveals Cytotoxicity Bases in Normal Cells

NMR spectroscopy and antioxidant activity of flavanones and flavones isolated from Chromolaena tacotana (Klatt) R.M. King & H. Rob.

Rapid evolution of the Helicobacter pylori AlpA adhesin in a high gastric cancer risk region from Colombia