Desarrollo de un método de análisis por cromatografía líquida de alta eficiencia (HPLC-DAD) para la caracterización de especies de la familia Asteraceae
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Angulo Alvarez, Sussan Xilena | 2019
In Colombia there are more than 27,000 species of plants and most of them, their composition or biological activity are unknown, because taxonomic identification is not always easy, since it requires a botanical professional.
Some species of the genera Chromolaena, Gnaphalium, Achyrocline and Gamochaeta, of the family Asteraceae (vira-viras); They are recognized in Colombia for their therapeutic applications in various pathologies, so some research conducted in recent years, including of the research group PRONAUDCA, of the University of Applied and Environmental Sciences, have focused on this family and identifies flavonoids as some of the potentially active compounds.
This metabolites, due to this, have become one of the most studied groups of molecules, both in vivo and in vitro, finding a high antioxidant, anti-inflammatory, antimicrobial, cardioprotective and antineoplastic activity.
In accordance with the above, the present work was based on the development of an analytical method by high performance liquid chromatography (HPLC-DAD) for the characterization of species belonging to this family, which allows to separate and identify the composition of plant extracts, creating fingerprints that become a tool that allows faster identification of these species
The development of the method consisted of a gradient with a flow of 1.1 ml/min; a mobile phase composed of methanol- acetic acid (2%) in water, at different concentrations; a C18 reverse phase column and a LaChrom Elite liquid chromatograph coupled to a UV-VIS UV detector.
Thanks to this, the separation of 12 flavonoids present in the family of asteraceae was achieved and characterized. In addition, an extract of the species Achyrocline Saturaioide was analized, finding 4 of the flavonoids worked in this investigation, which were identified thanks to the comparison in the retention times and the absorbance spectra obtained by using the diode array detector.
Therefore, it is recommended to carry out more tests with these extracts in future research to advance in the creation of a fingerprint library for the characterization of these species through the presence of reproducible patterns. Additionally, the validation of the method was advanced with the realization of the linearity parameter
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