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Bovine Leukemia Virus Gene Segment Detected in Human Breast Tissue

Mesa, Giovanna; Ulloa, Juan Carlos; Uribe, Ana María; Gutiérrez, María Fernanda (2013)

Background: Bovine Leukemia Virus (BLV) is known by infections in bovine cattle and produce, in 30% of infected animals, persistent lymphocytosis significantly impacts the beef industry. It has been proposed that this virus ...
Genomas virales fragmentados sugieren contaminación para aguas de consumo humano

Lenis, Cristina; López, Jazmín; Ulloa, Juan Carlos; Olaya, Nury; Gutiérrez, María Fernanda (2012)

Objetivo:Determinar la presencia de rotavirus y norovirus en agua para consumo humano en una localidad de Bogotá. Materiales y métodos:Se recolectaron ocho muestras de agua por semana en ocho lugares diferentes del predio ...
Técnica no convencional de extracción de ADN a partir de tejido embebido en parafina para uso en la reacción en cadena de la polimerasa

Ulloa, Juan Carlos; Gutiérrez, María Fernanda; Meza Barreto, Giovanna (Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2013, 2013-01)

The paraffin-embedded tissues are samples resting in the pathology banks, storing important genetic information, useful for retrospective studies of epidemiology and molecular pathology and ...

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Bovine Leukemia Virus Gene Segment Detected in Human Breast Tissue
...
Mesa, Giovanna | 2013

Background: Bovine Leukemia Virus (BLV) is known by infections in bovine cattle and produce, in 30% of infected animals, persistent lymphocytosis significantly impacts the beef industry. It has been proposed that this virus could be transmitted to humans and be present in cases of breast cancer. Aim: to determine the presence of 380 bp of gag gene segment of BLV in paraffin-embedded breast tissue. Study Design: Control-case study. Methodology: 106 tissue sam- ples were collected. 53 were cancer positive samples and 53 were negative samples for this pathology. After dewaxing tissues, DNA was extracted, amplified and sequenced. Phylogenetic analysis was done in order to verify BLV gene seg- ment, presence and origin. Results: 43 samples were positive (40.5%) for BLV segment. In the case group this segment was found in 35.8% of the samples and in the control group, BLV presented in 45.2% of the samples. Phylogenetic analysis confirmed BLV presence and had shown a high homology between amplified gene sequences obtained from human breast tissues and those coming from bovine cattle with leukosis reported by GenBank. Conclusion: The pres- ence of BLV genes in humans and its location in breast tissue can be confirmed, however, it should be clarified as a possible promoter of malignancy processes on this tissue.

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Genomas virales fragmentados sugieren contaminación para aguas de consumo humano
...
Lenis, Cristina | 2012

Objetivo:Determinar la presencia de rotavirus y norovirus en agua para consumo humano en una localidad de Bogotá. Materiales y métodos:Se recolectaron ocho muestras de agua por semana en ocho lugares diferentes del predio en estudio. Para determinar la presencia del genoma viral, las muestras se ultrafiltraron y se hizo detección viral por medio de la técnica RT-PCR, amplificando un segmento del gen VP6 del rotavirus y un segmento del ORF2 del norovirus. Este estudio se llevó a cabo en una institución universitaria ubicada en una zona periurbana cercana a Bogotá, a 45 km del acueducto que surte de agua al predio. La zona se encuentra rodeada por fincas con ganado lechero, centros comerciales y cuatro cementerios. El agua se recolectó en recipientes de polipropileno. En total, fueron 64 muestras de cinco litros de agua cada una. Resultados: La presencia viral fue de 12,5%, encontrándose rotavirus en cuatro y norovirus en cuatro de las 64 muestras colectadas. Además de presentar la importancia de la presencia viral en agua para consumo humano, en este documento se discute el significado que tiene el encontrar solo segmentos virales y no la partícula viral completa e infecciosa.

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Técnica no convencional de extracción de ADN a partir de tejido embebido en parafina para uso en la reacción en cadena de la polimerasa
...
Ulloa, Juan Carlos | 2013-01

The paraffin-embedded tissues are samples resting in the pathology banks, storing important genetic information, useful for retrospective studies of epidemiology and molecular pathology and diagnosis of diseases in humans and animals. Among the methods described for extracting DNA from tissues in paraffin, few are successful in obtaining the genetic material which can be amplified above 200bp fragments by the PCR technique. This paper describes the experience of U.D.C.A ́s molecular biology laboratory, related to the application of a methodology recently used to extract DNA from paraffin-embedded tissue and which permits to be optimal amplified by PCR of a fragment to 600pb. Two techniques were used, extensively referenced to these samples, such as the phenol extraction protocol and the commercial kit from QIAGEN®, and also a third commonly used method for extracting DNA from blood samples, but not for tissue in paraffin, the technique of extraction or salting out salts. The extracted DNA amplifiable ability was evidenced to obtain a fragment of 600pb establishing a human gene. The results showed that extraction with phenol and commercial kit from QIAGEN ® achieved amplifications of a 21%, while the salt extraction method a 91%. This work reveals that an unconventional technique of DNA extraction from paraffin-embedded tissue can offer better performance than traditional methodologies.

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Browsing by Author "Gutiérrez, María Fernanda"

Bovine Leukemia Virus Gene Segment Detected in Human Breast Tissue ﻿

Genomas virales fragmentados sugieren contaminación para aguas de consumo humano ﻿

Técnica no convencional de extracción de ADN a partir de tejido embebido en parafina para uso en la reacción en cadena de la polimerasa ﻿

Envíos recientes

Bovine Leukemia Virus Gene Segment Detected in Human Breast Tissue

Genomas virales fragmentados sugieren contaminación para aguas de consumo humano

Técnica no convencional de extracción de ADN a partir de tejido embebido en parafina para uso en la reacción en cadena de la polimerasa

Bovine Leukemia Virus Gene Segment Detected in Human Breast Tissue

Genomas virales fragmentados sugieren contaminación para aguas de consumo humano

Técnica no convencional de extracción de ADN a partir de tejido embebido en parafina para uso en la reacción en cadena de la polimerasa