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Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections

Araujo, Zaida; de Waard, Jacobus H.; Camargo, Milena; Lopez Ramos, Juan Ernesto; Fernández de Larrea, Carlos; Vanegas, Magnolia; Patarroyo, Manuel-Alfonso (2022)

Diagnosis and treatment of active tuberculosis (ATB) as well as latent tuberculosis infection (LTBI) are required for efective tuberculosis (TB) control, especially in TB endemic area. The usefulness of conventional tests ...
ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis

Araujo, Zaida; Fernández de Larrea, Carlos; López, Diana; Isern-Kebschull, Jaime; de Waard, Jacobus H.; Hagel, Isabel; Camargo, Milena; Vanegas, Magnolia ; Patarroyo, Manuel-Alfonso (2021)

Background. Tuberculosis (TB) is being underdetected in children as most are smear-negative. This work was aimed at evaluating ESAT-6 and Ag85A synthetic peptides’ serodiagnostic potential for diagnosing children having a ...

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Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections
...
Araujo, Zaida | 2022

Diagnosis and treatment of active tuberculosis (ATB) as well as latent tuberculosis infection (LTBI) are required for efective tuberculosis (TB) control, especially in TB endemic area. The usefulness of conventional tests to distinguish between ATB and LTBI has remained challenging. The present study was aimed to demonstrate the usefulness of the serological response to synthetic peptides from Mycobacterium tuberculosis (Mtb) antigens for discrimination between ATB and LTBI in Warao Amerindians. Serum IgG antibody levels were measured by the indirect ELISA assay using 22 designed and synthesized peptides derived from immunogenic Mtb ESAT-6 and Ag85A proteins. A total of 211 adult Warao Amerindians were included; cases with active TB (ATB, n=75), latent TB infection (LTBI, n=85) and non-infected (NI, n=51). The approach’s diagnostic information was compared using receiver operating characteristic (ROC) curves. For ATB diagnostic performance between ATB and NI; ESAT-6; P-12037 had 100% of sensitivity (AUC=0.812; 0.733 to 0.891 95% CI); and Ag85A; P-10997 had 100% of specifcity (AUC=0.691; 0.597 to 0.785 95% CI); and ATB and LTBI; Ag85A; P-29878 had 100% of sensitivity (AUC=0.741; 0.666–0.817 95% CI), and P-29879 had 99% of specifcity (AUC=0.679; 0.593–0.765 95% CI). While that ESAT-6 P-12037 also allowed diferentiation between LTBI and NI or healthy ones. It had 98.8% of sensitivity and 98.0% of specifcity (AUC=0.640; 0.545–0.735 95% CI). The potential of combination-antigen immunoassays with peptides could discriminate between Warao Amerindians with ATB, LTBI and NI. Further validation of this approach could lead to developing a complementary tool for rapid diagnosis of TB infections.

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ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis
...
Araujo, Zaida | 2021

Background. Tuberculosis (TB) is being underdetected in children as most are smear-negative. This work was aimed at evaluating ESAT-6 and Ag85A synthetic peptides’ serodiagnostic potential for diagnosing children having a clinical suspicion of TB. Methods. The study involved 438 children: 77 Creole nonindigenous (13 suspected of having TB and 64 healthy ones) and 361 Warao indigenous children (39 suspected of TB and 322 healthy children). The approach’s diagnostic information was compared using operational characteristics and receiver-operating characteristic (ROC) curves. Results. Ag85A P-29879 had 94.6% sensitivity (: 0.651 to 0.819 95% CI) in indigenous children. ESAT-6 P-12036 and P-12037 had 100% and 92.3% of sensitivity (: 0.929: 0.846 to 0.975 95% CI and 0.791: 63.9 to 98.7 95% CI, respectively) in Creole children. ESAT-6 peptides also allowed a differentiation between children with TB and healthy ones. Conclusions. Further validation of this approach could lead to developing a complementary tool for rapid TB diagnosis in children.

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Browsing by Author "Fernández de Larrea, Carlos"

Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections ﻿

ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis ﻿

Envíos recientes

Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections

ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis

Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections

ESAT-6 and Ag85A Synthetic Peptides as Candidates for an Immunodiagnostic Test in Children with a Clinical Suspicion of Tuberculosis