Producción por la levadura saccharomyces cerevisiae de gamma-decalactona, principal componente del aroma a melocotón
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Cárdenas, Claudia | 2006-07
A study to assess the gamma-decalactone pro-duction from castor oil and ricinoleic acid by biotransformation using the yeast Saccharomyces cerevisiae and Rhodotorula glutinis was carried out by the Pontificia Universidad Javeriana. The results showed higher production of lactone by S. cerevisiae in the médium with ricinoleic acid as substrate. In this research the médium was refor-mulated with the main aim to increase the deca-lactone production. To reformulate and check the gamma's decalatone production, five steps were taken. The first one was the development of seve-ral media to choose the most uniform and trans-parent one. Afterwards the appropriate amount of yeast for inoculation and the right temperature and pH was established. Then into the chosen médium 1% and 2% of ricinoleic acid was incorporated, with and without the tensioactive Tween 80. Finallv the veast was inoculated J J within the four media to measure the quantity of decalactone gamma produced and determine the viability or alive population. The results showed that the gamma decalactone production increased with the médium that included Tween 80, with ricinoleic acid at 2% as substrate.
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